Optogenetic regulation of embryo implantation in mice using photoactivatable CRISPR-Cas9

Tomoka Takao, Moritoshi Sato, Tetsuo Maruyama

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Embryo implantation is achieved upon successful interaction between a fertilized egg and receptive endometrium and is mediated by spatiotemporal expression of implantation-associated molecules including leukemia inhibitory factor (LIF). Here we demonstrate, in mice, that LIF knockdown via a photoactivatable CRISPRCas9 gene editing system and illumination with a light-emitting diode can spatiotemporally disrupt fertility. This system enables dissection of spatiotemporal molecular mechanisms associated with embryo implantation and provides a therapeutic strategy for temporal control of reproductive functions in vivo.

本文言語English
ページ(範囲)28579-28581
ページ数3
ジャーナルProceedings of the National Academy of Sciences of the United States of America
117
46
DOI
出版ステータスPublished - 2020 11 17

ASJC Scopus subject areas

  • General

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