Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support

Kazuhiro Katayama, Yoh Dobashi, Masatoshi Kitagawa, Soichiro Kamekura, Masataka Kawai, Yuichi Kadoya, Toru Kameya

研究成果: Article査読

18 被引用数 (Scopus)

抄録

The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwent apoptosis, which was not associated with G1-arrest. Cdk4 kinase activity was significantly higher in cdk4-, or cyclin D1-expressing cells. Furthermore, induction of apoptosis by cdk4 was abrogated by co-transfection of p16INK4, or dominant negative cdk4. These results suggest that upregulation of cdk4 kinase activity is a primary and critical mediator of apoptosis in PC12 cells under physiological conditions.

本文言語English
ページ(範囲)382-388
ページ数7
ジャーナルFEBS Letters
509
3
DOI
出版ステータスPublished - 2001 12 14

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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