Overexpression of interleukin 21 induces expansion of hematopoietic progenitor cells

Katsutoshi Ozaki, Ai Hishiya, Keiko Hatanaka, Hideaki Nakajima, Gang Wang, Patrick Hwu, Toshio Kitamura, Keiya Ozawa, Warren J. Leonard, Tetsuya Nosaka

研究成果: Article

17 引用 (Scopus)

抜粋

The interleukin 21 (IL-21) receptor is expressed on T-cells, B-cells, and natural killer cells, and IL-21 is critical for regulating immunoglobulin production in vivo in cooperation with IL-4. So far, however, little is known about a role for IL-21 outside the immune system. We investigated the effect of IL-21 on hematopoiesis in vivo by using the hydrodynamics gene-delivery method. Overexpression of IL-21 increases Sca-1+ cells in the periphery and spleen. It also increases the numbers of C-Kit+, Sca-1+, and lineage-/low (KSL) cells and colony-forming units-granulocyte- macrophage (CFU-GM) in the spleen, indicating the expansion of hematopoietic progenitor cells. We found that even in RAG2-/- mice, which lack mature T-cells and B-cells, IL-21 induced an increase in KSL cells and CFU-GM in the spleen. These results demonstrate that IL-21 can induce the expansion of hematopoietic progenitor cells in vivo, even in the absence of mature T-cells and B-cells.

元の言語English
ページ(範囲)224-230
ページ数7
ジャーナルInternational journal of hematology
84
発行部数3
DOI
出版物ステータスPublished - 2006 10 1

    フィンガープリント

ASJC Scopus subject areas

  • Hematology

これを引用

Ozaki, K., Hishiya, A., Hatanaka, K., Nakajima, H., Wang, G., Hwu, P., Kitamura, T., Ozawa, K., Leonard, W. J., & Nosaka, T. (2006). Overexpression of interleukin 21 induces expansion of hematopoietic progenitor cells. International journal of hematology, 84(3), 224-230. https://doi.org/10.1532/IJH97.06036