Oxidative stress on mitochondria and cell membrane of cultured rat hepatocytes and perfused liver exposed to ethanol

I. Kurose, H. Higuchi, Shinzo Kato, S. Miura, N. Watanabe, Y. Kamegaya, K. Tomita, M. Takaishi, Y. Horie, M. Fukuda, K. Mizukami, H. Ishii

研究成果: Article

104 引用 (Scopus)

抄録

Background and Aims: The precise pathogenic significance of oxidative injury in the evolution of alcohol-induced liver disease is still obscure. The present report was designed to investigate whether ethanol alters the production of active oxidants and biological activities of hepatocytes. Methods: The following parameters in rat hepatocytes were investigated by using fluorescence probes in vitro and ex vivo: (1) mitochondrial membrane potential and membrane permeability transition, (2) oxygen radicals generation, (3) membrane barrier function, and (4) glutathione level. Results: Ethanol (50 mmol/l) increased oxidative stress in hepatocytes and subsequently induced an increased mitochondrial permeability transition and a decreased membrane potential. These ethanol-induced alterations were attenuated by an inhibitor of alcohol dehydrogenase and an intracellular oxidant scavenger, whereas they were enhanced by diethyl maleic acid, a glutathione depletor. Ethanol plus diethyl maleic acid but not ethanol alone increased the number of hepatocytes with membrane barrier dysfunction. A continuous infusion of ethanol (50 mmol/l) increased oxidative stress and decreased mitochondrial membrane potential in the pericentral area of isolated perfused rat liver. Conclusions: Active oxidants generated during ethanol metabolism increase mitochondrial permeability transition and modulate mitochondrial energy synthesis in hepatocytes. Reduction of glutathione level enhances mitochondrial dysfunction and impairs membrane barrier function of hepatocytes.

元の言語English
ページ(範囲)1331-1343
ページ数13
ジャーナルGastroenterology
112
発行部数4
DOI
出版物ステータスPublished - 1997

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Hepatocytes
Mitochondria
Oxidative Stress
Ethanol
Cell Membrane
Liver
Oxidants
Glutathione
Permeability
Membranes
Mitochondrial Membrane Potential
Alcohol Dehydrogenase
Membrane Potentials
Liver Diseases
Reactive Oxygen Species
Fluorescence
Alcohols
Wounds and Injuries

ASJC Scopus subject areas

  • Gastroenterology

これを引用

Oxidative stress on mitochondria and cell membrane of cultured rat hepatocytes and perfused liver exposed to ethanol. / Kurose, I.; Higuchi, H.; Kato, Shinzo; Miura, S.; Watanabe, N.; Kamegaya, Y.; Tomita, K.; Takaishi, M.; Horie, Y.; Fukuda, M.; Mizukami, K.; Ishii, H.

:: Gastroenterology, 巻 112, 番号 4, 1997, p. 1331-1343.

研究成果: Article

Kurose, I, Higuchi, H, Kato, S, Miura, S, Watanabe, N, Kamegaya, Y, Tomita, K, Takaishi, M, Horie, Y, Fukuda, M, Mizukami, K & Ishii, H 1997, 'Oxidative stress on mitochondria and cell membrane of cultured rat hepatocytes and perfused liver exposed to ethanol', Gastroenterology, 巻. 112, 番号 4, pp. 1331-1343. https://doi.org/10.1016/S0016-5085(97)70147-1
Kurose, I. ; Higuchi, H. ; Kato, Shinzo ; Miura, S. ; Watanabe, N. ; Kamegaya, Y. ; Tomita, K. ; Takaishi, M. ; Horie, Y. ; Fukuda, M. ; Mizukami, K. ; Ishii, H. / Oxidative stress on mitochondria and cell membrane of cultured rat hepatocytes and perfused liver exposed to ethanol. :: Gastroenterology. 1997 ; 巻 112, 番号 4. pp. 1331-1343.
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T1 - Oxidative stress on mitochondria and cell membrane of cultured rat hepatocytes and perfused liver exposed to ethanol

AU - Kurose, I.

AU - Higuchi, H.

AU - Kato, Shinzo

AU - Miura, S.

AU - Watanabe, N.

AU - Kamegaya, Y.

AU - Tomita, K.

AU - Takaishi, M.

AU - Horie, Y.

AU - Fukuda, M.

AU - Mizukami, K.

AU - Ishii, H.

PY - 1997

Y1 - 1997

N2 - Background and Aims: The precise pathogenic significance of oxidative injury in the evolution of alcohol-induced liver disease is still obscure. The present report was designed to investigate whether ethanol alters the production of active oxidants and biological activities of hepatocytes. Methods: The following parameters in rat hepatocytes were investigated by using fluorescence probes in vitro and ex vivo: (1) mitochondrial membrane potential and membrane permeability transition, (2) oxygen radicals generation, (3) membrane barrier function, and (4) glutathione level. Results: Ethanol (50 mmol/l) increased oxidative stress in hepatocytes and subsequently induced an increased mitochondrial permeability transition and a decreased membrane potential. These ethanol-induced alterations were attenuated by an inhibitor of alcohol dehydrogenase and an intracellular oxidant scavenger, whereas they were enhanced by diethyl maleic acid, a glutathione depletor. Ethanol plus diethyl maleic acid but not ethanol alone increased the number of hepatocytes with membrane barrier dysfunction. A continuous infusion of ethanol (50 mmol/l) increased oxidative stress and decreased mitochondrial membrane potential in the pericentral area of isolated perfused rat liver. Conclusions: Active oxidants generated during ethanol metabolism increase mitochondrial permeability transition and modulate mitochondrial energy synthesis in hepatocytes. Reduction of glutathione level enhances mitochondrial dysfunction and impairs membrane barrier function of hepatocytes.

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