Paf1 complex homologues are required for Notch-regulated transcription during somite segmentation

Takashi Akanuma, Sumito Koshida, Akinori Kawamura, Yasuyuki Kishimoto, Shinji Takada

研究成果: Article

28 引用 (Scopus)

抄録

Members of the yeast polymerase-associated factor 1 (Paf1) complex, which is composed of at least five components (Paf1, Rtf1, Cdc73, Leo1 and Ctr9), are conserved from yeast to humans. Although these proteins have been implicated in RNA polymerase II-mediated transcription, their roles in vertebrate development have not been explained. Here, we show that a zebrafish mutant with a somite segmentation defect is deficient in rtf1. In addition, embryos deficient in rtf1 or ctr9 show abnormal development of the heart, ears and neural crest cells. rtf1 is required for correct RNA levels of the Notch-regulated genes her1, her7 and deltaC, and also for Notch-induced her1 expression in the presomitic mesoderm. Furthermore, the phenotype observed in rtf1-deficient mutants is enhanced by an additional deficiency in mind bomb, which encodes an effector of Notch signalling. Therefore, zebrafish homologues of the yeast Paf1 complex seem to preferentially affect a subset of genes, including Notch-regulated genes, during embryogenesis.

元の言語English
ページ(範囲)858-863
ページ数6
ジャーナルEMBO Reports
8
発行部数9
DOI
出版物ステータスPublished - 2007 9
外部発表Yes

Fingerprint

Somites
Transcription
Yeast
Genes
Yeasts
Zebrafish
Bombs
RNA Polymerase II
Neural Crest
Mesoderm
Embryonic Development
Ear
Vertebrates
Embryonic Structures
RNA
Phenotype
Defects
Proteins

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

これを引用

Paf1 complex homologues are required for Notch-regulated transcription during somite segmentation. / Akanuma, Takashi; Koshida, Sumito; Kawamura, Akinori; Kishimoto, Yasuyuki; Takada, Shinji.

:: EMBO Reports, 巻 8, 番号 9, 09.2007, p. 858-863.

研究成果: Article

Akanuma, Takashi ; Koshida, Sumito ; Kawamura, Akinori ; Kishimoto, Yasuyuki ; Takada, Shinji. / Paf1 complex homologues are required for Notch-regulated transcription during somite segmentation. :: EMBO Reports. 2007 ; 巻 8, 番号 9. pp. 858-863.
@article{7fed89d8bac44bdba5fa1943677ec873,
title = "Paf1 complex homologues are required for Notch-regulated transcription during somite segmentation",
abstract = "Members of the yeast polymerase-associated factor 1 (Paf1) complex, which is composed of at least five components (Paf1, Rtf1, Cdc73, Leo1 and Ctr9), are conserved from yeast to humans. Although these proteins have been implicated in RNA polymerase II-mediated transcription, their roles in vertebrate development have not been explained. Here, we show that a zebrafish mutant with a somite segmentation defect is deficient in rtf1. In addition, embryos deficient in rtf1 or ctr9 show abnormal development of the heart, ears and neural crest cells. rtf1 is required for correct RNA levels of the Notch-regulated genes her1, her7 and deltaC, and also for Notch-induced her1 expression in the presomitic mesoderm. Furthermore, the phenotype observed in rtf1-deficient mutants is enhanced by an additional deficiency in mind bomb, which encodes an effector of Notch signalling. Therefore, zebrafish homologues of the yeast Paf1 complex seem to preferentially affect a subset of genes, including Notch-regulated genes, during embryogenesis.",
author = "Takashi Akanuma and Sumito Koshida and Akinori Kawamura and Yasuyuki Kishimoto and Shinji Takada",
year = "2007",
month = "9",
doi = "10.1038/sj.embor.7401045",
language = "English",
volume = "8",
pages = "858--863",
journal = "EMBO Reports",
issn = "1469-221X",
publisher = "Nature Publishing Group",
number = "9",

}

TY - JOUR

T1 - Paf1 complex homologues are required for Notch-regulated transcription during somite segmentation

AU - Akanuma, Takashi

AU - Koshida, Sumito

AU - Kawamura, Akinori

AU - Kishimoto, Yasuyuki

AU - Takada, Shinji

PY - 2007/9

Y1 - 2007/9

N2 - Members of the yeast polymerase-associated factor 1 (Paf1) complex, which is composed of at least five components (Paf1, Rtf1, Cdc73, Leo1 and Ctr9), are conserved from yeast to humans. Although these proteins have been implicated in RNA polymerase II-mediated transcription, their roles in vertebrate development have not been explained. Here, we show that a zebrafish mutant with a somite segmentation defect is deficient in rtf1. In addition, embryos deficient in rtf1 or ctr9 show abnormal development of the heart, ears and neural crest cells. rtf1 is required for correct RNA levels of the Notch-regulated genes her1, her7 and deltaC, and also for Notch-induced her1 expression in the presomitic mesoderm. Furthermore, the phenotype observed in rtf1-deficient mutants is enhanced by an additional deficiency in mind bomb, which encodes an effector of Notch signalling. Therefore, zebrafish homologues of the yeast Paf1 complex seem to preferentially affect a subset of genes, including Notch-regulated genes, during embryogenesis.

AB - Members of the yeast polymerase-associated factor 1 (Paf1) complex, which is composed of at least five components (Paf1, Rtf1, Cdc73, Leo1 and Ctr9), are conserved from yeast to humans. Although these proteins have been implicated in RNA polymerase II-mediated transcription, their roles in vertebrate development have not been explained. Here, we show that a zebrafish mutant with a somite segmentation defect is deficient in rtf1. In addition, embryos deficient in rtf1 or ctr9 show abnormal development of the heart, ears and neural crest cells. rtf1 is required for correct RNA levels of the Notch-regulated genes her1, her7 and deltaC, and also for Notch-induced her1 expression in the presomitic mesoderm. Furthermore, the phenotype observed in rtf1-deficient mutants is enhanced by an additional deficiency in mind bomb, which encodes an effector of Notch signalling. Therefore, zebrafish homologues of the yeast Paf1 complex seem to preferentially affect a subset of genes, including Notch-regulated genes, during embryogenesis.

UR - http://www.scopus.com/inward/record.url?scp=35548950642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35548950642&partnerID=8YFLogxK

U2 - 10.1038/sj.embor.7401045

DO - 10.1038/sj.embor.7401045

M3 - Article

C2 - 17721442

AN - SCOPUS:35548950642

VL - 8

SP - 858

EP - 863

JO - EMBO Reports

JF - EMBO Reports

SN - 1469-221X

IS - 9

ER -