Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent phosphorylation

Masahiro Iguchi, Yuki Kujuro, Kei Okatsu, Fumika Koyano, Hidetaka Kosako, Mayumi Kimura, Norihiro Suzuki, Shinichiro Uchiyama, Keiji Tanaka, Noriyuki Matsuda

研究成果: Article査読

137 被引用数 (Scopus)

抄録

Background: Parkin is a ubiquitin ligase activated by a decrease in the mitochondrial membrane potential (Δ Φm). However, details regarding its mechanism remain limited. Results: PINK1-dependent phosphorylation of Parkin at Ser-65 following dissipation of Δ Φm triggers ubiquitin-ester transfer by the RING2 domain of Parkin to Cys-431. Conclusion: Parkin catalyzes trans- (ubiquitin-thioester)ification upon PINK1-dependent phosphorylation. Significance: The molecular process of Parkin-catalyzed ubiquitylation has been determined.

本文言語English
ページ(範囲)22019-22032
ページ数14
ジャーナルJournal of Biological Chemistry
288
30
DOI
出版ステータスPublished - 2013 7 26

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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