Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease

Rosario Sanchez-Pernaute, Hyojin Lee, Michaela Patterson, Casper Reske-Nielsen, Takahito Yoshizaki, Kai C. Sonntag, Lorenz Studer, Ole Isacson

研究成果: Article査読

64 被引用数 (Scopus)

抄録

The identity and functional potential of dopamine neurons derived in vitro from embryonic stem cells are critical for the development of a stem cell-based replacement therapy for Parkinson's disease. Using a parthenogenetic primate embryonic stem cell line, we have generated dopamine neurons that display persistent expression of midbrain regional and cell-specific transcription factors, which establish their proper identity and allow for their survival. We show here that transplantation of parthenogenetic dopamine neurons restores motor function in hemi-parkinsonian, 6-hydroxy-dopamine-lesioned rats. Exposure to Wnt5a and fibroblast growth factors (FGF) 20 and 2 at the final stage of in vitro differentiation enhanced the survival of dopamine neurons and, correspondingly, the extent of motor recovery of transplanted animals. Importantly for future development of clinical applications, dopamine neurons were post-mitotic at the time of transplantation and there was no tumour formation. These data provide proof for the concept that parthenogenetic stem cells are a suitable source of functional neurons for therapeutic applications.

本文言語English
ページ(範囲)2127-2139
ページ数13
ジャーナルBrain
131
8
DOI
出版ステータスPublished - 2008 8

ASJC Scopus subject areas

  • 臨床神経学

フィンガープリント

「Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル