Participation of reactive oxygen species in PGF(2α)-induced apoptosis in rat luteal cells

M. Tanaka, T. Miyazaki, S. Tanigaki, K. Kasai, Kazuhiro Minegishi, K. Miyakoshi, H. Ishimoto, Y. Yoshimura

研究成果: Article査読

47 被引用数 (Scopus)

抄録

Prostaglandin F(2α) (PGF(2α)) is implicated in the process of luteal regression in many species. Treatment of rat luteal tissue with PGF(2α) increases the generation of reactive oxygen species. Since reactive oxygen species have been implicated in apoptosis, the present study was undertaken to determine whether reactive oxygen species play a role in the PGF(2α)-induced apoptosis of rat luteal cells. Rat luteal cells were loaded with 6- carboxy-2,7,-dichlorodihydro-fluorescein (CDCFH) diacetate, di (acetomethyl ester), which can be oxidized by reactive oxygen species to yield CDCF, a fluorescent molecule, and the cells were treated with different doses of PGF(2α). Incubation with 100 μmol PGF(2α) I-1 induced an increase in CDCF fluorescence (P<0.05). Treatment of cells with PGF(2α) for 48 h in serum-free medium induced a dose-dependent increase in cell death, and these cells exhibited the morphological characteristics typical of apoptosis, including condensed or fragmented nuclei and fragmentation of internucleosomal DNA. Pretreatment of these cells with ascorbic acid, N,N'-dimethylthiourea, or superoxide dismutase, which acts as an antioxidant or a radical scavenger, prevented the PGF(2α)-induced apoptosis. These results demonstrate that PGF(2α) produces reactive oxygen species and induces apoptosis in rat luteal cells, indicating that the reactive oxygen species may induces apoptosis cell death during luteolysis.

本文言語English
ページ(範囲)239-245
ページ数7
ジャーナルJournal of Reproduction and Fertility
120
2
DOI
出版ステータスPublished - 2000 1 1

ASJC Scopus subject areas

  • 生理学
  • 胎生学
  • 分子生物学
  • 産婦人科学
  • 発生生物学

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