PCCX1, a novel DNA-binding protein with PHD finger and CXXC domain, is regulated by proteolysis

Tadahiro Fujino, Mayumi Hasegawa, Shinsuke Shibata, Taishiro Kishimoto, Shin Ichiro Imai, Toshiya Takano

研究成果: Article査読

4 被引用数 (Scopus)

抄録

We identified a novel gene PCCX1 that encoded a nuclear protein carrying a PHD finger, a CXXC domain, and an acidic region. The CXXC domain was found to be sufficient for binding to DNA. The acidic region exhibited a high transactivation ability, but the full-length protein was inactive due to regions which inhibited the acidic region, including the C-terminal region. We examined the expression of PCCX1 during cellular aging and immortalization of SV40-transformed human fibroblasts. PCCX1 mRNA was expressed constitutively through stages of cellular aging and immortalization, but at the protein level, a shorter form lacking the C-terminal region appeared as the cells approached crisis. These results suggested that PCCX1 was activated by proteolytic cleavage, which removed the C-terminal inhibitory region. (C) 2000 Academic Press.

本文言語English
ページ(範囲)305-310
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
271
2
DOI
出版ステータスPublished - 2000 5月 10
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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