To identify peptides with selective affinity for stereoregular isotactic poly(methyl methacrylate) (it-PMMA), the phage display selection using a heptamer random peptide library was carried out. After five rounds of biopanning, several phage clones with high specific affinity to it-PMMA were selected. As a result of DNA sequencing, nine kinds of it-PMMA binding phage clones were isolated. The binding affinity and specificity of these phage clones were quantitatively investigated by an enzyme-linked immunosorbent assay. These clones not only bound to it-PMMA greater than a phage library, but showed the specific binding to it-PMMA rather than syndiotactic and atactic PMMAs.
|出版ステータス||Published - 2005 12月 1|
|イベント||54th SPSJ Symposium on Macromolecules - Yamagata, Japan|
継続期間: 2005 9月 20 → 2005 9月 22
|Other||54th SPSJ Symposium on Macromolecules|
|Period||05/9/20 → 05/9/22|
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