Peptides bound to stereoregular PMMAs

Toshiki Sawada; Hisao Matsuno; Teruhiko Matsubara; Toshinori Sato; Tatsuki Kitayama; Takeshi Serizawa

Peptides bound to stereoregular PMMAs

Toshiki Sawada, Hisao Matsuno, Teruhiko Matsubara, Toshinori Sato, Tatsuki Kitayama, Takeshi Serizawa

生命情報学科

研究成果: Paper › 査読

抄録

To identify peptides with selective affinity for stereoregular isotactic poly(methyl methacrylate) (it-PMMA), the phage display selection using a heptamer random peptide library was carried out. After five rounds of biopanning, several phage clones with high specific affinity to it-PMMA were selected. As a result of DNA sequencing, nine kinds of it-PMMA binding phage clones were isolated. The binding affinity and specificity of these phage clones were quantitatively investigated by an enzyme-linked immunosorbent assay. These clones not only bound to it-PMMA greater than a phage library, but showed the specific binding to it-PMMA rather than syndiotactic and atactic PMMAs.

本文言語	English
ページ	4867-4868
ページ数	2
出版ステータス	Published - 2005 12月 1
イベント	54th SPSJ Symposium on Macromolecules - Yamagata, Japan 継続期間: 2005 9月 20 → 2005 9月 22

Other

Other	54th SPSJ Symposium on Macromolecules
国/地域	Japan
City	Yamagata
Period	05/9/20 → 05/9/22

ASJC Scopus subject areas

工学（全般）

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引用スタイル

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title = "Peptides bound to stereoregular PMMAs",

abstract = "To identify peptides with selective affinity for stereoregular isotactic poly(methyl methacrylate) (it-PMMA), the phage display selection using a heptamer random peptide library was carried out. After five rounds of biopanning, several phage clones with high specific affinity to it-PMMA were selected. As a result of DNA sequencing, nine kinds of it-PMMA binding phage clones were isolated. The binding affinity and specificity of these phage clones were quantitatively investigated by an enzyme-linked immunosorbent assay. These clones not only bound to it-PMMA greater than a phage library, but showed the specific binding to it-PMMA rather than syndiotactic and atactic PMMAs.",

keywords = "Peptide, Phage display, Poly(methyl methacrylate), Tacticity",

author = "Toshiki Sawada and Hisao Matsuno and Teruhiko Matsubara and Toshinori Sato and Tatsuki Kitayama and Takeshi Serizawa",

year = "2005",

month = dec,

day = "1",

language = "English",

pages = "4867--4868",

note = "54th SPSJ Symposium on Macromolecules ; Conference date: 20-09-2005 Through 22-09-2005",

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TY - CONF

T1 - Peptides bound to stereoregular PMMAs

AU - Sawada, Toshiki

AU - Matsuno, Hisao

AU - Matsubara, Teruhiko

AU - Sato, Toshinori

AU - Kitayama, Tatsuki

AU - Serizawa, Takeshi

PY - 2005/12/1

Y1 - 2005/12/1

N2 - To identify peptides with selective affinity for stereoregular isotactic poly(methyl methacrylate) (it-PMMA), the phage display selection using a heptamer random peptide library was carried out. After five rounds of biopanning, several phage clones with high specific affinity to it-PMMA were selected. As a result of DNA sequencing, nine kinds of it-PMMA binding phage clones were isolated. The binding affinity and specificity of these phage clones were quantitatively investigated by an enzyme-linked immunosorbent assay. These clones not only bound to it-PMMA greater than a phage library, but showed the specific binding to it-PMMA rather than syndiotactic and atactic PMMAs.

AB - To identify peptides with selective affinity for stereoregular isotactic poly(methyl methacrylate) (it-PMMA), the phage display selection using a heptamer random peptide library was carried out. After five rounds of biopanning, several phage clones with high specific affinity to it-PMMA were selected. As a result of DNA sequencing, nine kinds of it-PMMA binding phage clones were isolated. The binding affinity and specificity of these phage clones were quantitatively investigated by an enzyme-linked immunosorbent assay. These clones not only bound to it-PMMA greater than a phage library, but showed the specific binding to it-PMMA rather than syndiotactic and atactic PMMAs.

KW - Peptide

KW - Phage display

KW - Poly(methyl methacrylate)

KW - Tacticity

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M3 - Paper

AN - SCOPUS:33645578430

SP - 4867

EP - 4868

T2 - 54th SPSJ Symposium on Macromolecules

Y2 - 20 September 2005 through 22 September 2005

ER -

Peptides bound to stereoregular PMMAs

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Other

ASJC Scopus subject areas

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