Peptides derived from atrial natriuretic polypeptide precursor in human and monkey brains

Hiroshi Itoh, Kazuwa Nakao, Masashi Mukoyama, Kiminori Hosada, Shozo Shiono, Narito Morii, Takayuki Yamada, Akira Sugawara, Yoshiko Saito, Hiroshi Arai, Gotaro Shirakami, Hiroo Imura

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Using radio-immunoassays for the C-terminai sequence of a-atrial natriuretic polypeptide [atrial natriuretic factor (ANF)-(99-126)], α-ANP (17-28) [ANF-(115-126)], which corresponds to γ-human ANP [115-126; - γ -hAN P-(115-126)] and for the N-terminal sequence of γ -hANP [human ANF-(1-126)], γ -hANP-(1-25) [human ANF-(1-25)], we detected the parallel distribution of α-hANP-like immunoreactivity and - γ -hANP-(1- 25)-like immunoreactivity in the human and monkey brains, with the highest concentrations in the midbrain and the pons. High performance gel permeation chromatography coupled with the two radio-immunoassays and also with another radio-immunoassay using a monoclonal antibody against the N-terminal sequence of a-ANP revealed that γ -ANP is synthesized in the brain and cleaved into N-terminally deleted form(s) of α-ANP and 10-K N-terminal fragment(s) of γ -ANP, which coexist within the neuron. These results suggest that the post-translational processing of γ -ANP in the brain is different from that in the heart.

本文言語English
ページ(範囲)S309-S313
ジャーナルJournal of Hypertension, Supplement
6
4
出版ステータスPublished - 1988 12
外部発表はい

ASJC Scopus subject areas

  • 内科学
  • 生理学
  • 循環器および心血管医学

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