Perilipin+ embryonic preadipocytes actively proliferate along growing vasculatures for adipose expansion

Ki Yong Hong, Hosung Bae, Intae Park, Dae Young Park, Kyun Hoo Kim, Yoshiaki Kubota, Eui Sic Cho, Hail Kim, Ralf H. Adams, Ook Joon Yoo, Gou Young Koh

研究成果: Article査読

48 被引用数 (Scopus)

抄録

Despite the growing interest in adipose tissue as a therapeutic target of metabolic diseases, the identity of adipocyte precursor cells (preadipocytes) and the formation of adipose tissue during embryonic development are still poorly understood. Here, we clarified the identity and dynamic processes of preadipocytes in mouse white adipose tissue during embryogenesis through direct examination, lineage tracing and culture systems. Surprisingly, we found that lipid-lacking but perilipin+ or adiponectin+ proliferating preadipocytes started to emerge at embryonic day 16.5, and these cells underwent active proliferation until birth. Moreover, these preadipocytes resided as clusters and were distributed along growing adipose vasculatures. Importantly, the embryonic preadipocytes exhibited considerable coexpression of stem cell markers, such as CD24, CD29 and PDGFRα, and a small portion of preadipocytes were derived from PDGFRß+ mural cells, in contrast to the adult preadipocytes present in the stromal vascular fraction. Further analyses with in vitro and ex vivo culture systems revealed a stepwise but dynamic regulation of preadipocyte formation and differentiation during prenatal adipogenesis. To conclude, we unraveled the identity and characteristics of embryonic preadipocytes, which are crucial for the formation and expansion of adipose tissue during embryogenesis.

本文言語English
ページ(範囲)2623-2632
ページ数10
ジャーナルDevelopment (Cambridge)
142
15
DOI
出版ステータスPublished - 2015 8月 1

ASJC Scopus subject areas

  • 分子生物学
  • 発生生物学

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