Persistence of recipient-derived as well as donor-derived clones of cytomegalovirus pp65-specific cytotoxic T cells long after allogeneic hematopoietic stem cell transplantation

K. Terasako-Saito, H. Nakasone, Y. Tanaka, R. Yamazaki, M. Sato, K. Sakamoto, Y. Ishihara, K. Kawamura, Y. Akahoshi, J. Hayakawa, H. Wada, N. Harada, H. Nakano, K. Kameda, T. Ugai, R. Yamasaki, M. Ashizawa, S. I. Kimura, M. Kikuchi, A. TaniharaJ. Kanda, S. Kako, J. Nishida, Y. Kanda

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Background: Cytomegalovirus (CMV)-specific CD8+ cytotoxic T lymphocytes (CMV-CTLs) play a crucial role in preventing CMV disease. However, the actual in vivo dynamics of CMV-CTL clones after allogeneic hematopoietic stem cell transplantation (alloHCT) are still unclear. Methods: Using a single-cell T-cell receptor repertoire analysis, we monitored clones and chimerism of CMV-CTLs in 3 CMV-seropositive alloHCT recipients from CMV-seronegative donors, with or without CMV reactivation. Results: Nearly all of the CMV-CTLs during follow-up were CD45RA-CCR7- effector memory/CD45RA+CCR7- effector T cells, and were highly matured. In each case, the use of BV gene families was restricted, especially in BV5, 7, 28, and 29. Although no common predominant CMV-CTL clones were found, several shared motifs of complementarity-determining region-3 were identified among the 3 cases; QGA in all, TGE and TDT in Case 1 and Case 2, and RDRG in Case 2 and Case 3. In all cases, CMV-CTL clones that were detected for the first time after alloHCT persisted as the dominant clones. In Case 1, without CMV reactivation, recipient-derived CMV-CTLs exclusively persisted as a dominant clone, while all CMV-CTLs in the other 2 cases, with CMV reactivation, were donor derived. Conclusion: Clone monitoring and chimerism analyses should help to further clarify novel aspects of immuno-reconstitution after alloHCT.

本文言語English
ページ(範囲)930-940
ページ数11
ジャーナルTransplant Infectious Disease
16
6
DOI
出版ステータスPublished - 2014 12 1
外部発表はい

ASJC Scopus subject areas

  • Infectious Diseases
  • Transplantation

フィンガープリント 「Persistence of recipient-derived as well as donor-derived clones of cytomegalovirus pp65-specific cytotoxic T cells long after allogeneic hematopoietic stem cell transplantation」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル