Phenotypic screening system using three-dimensional (3D) culture models for natural product screening

Hikaru Suenaga, Noritaka Kagaya, Manabu Kawada, Daisuke Tatsuda, Toshiro Sato, Kazuo Shin-ya

研究成果: Review article査読

抄録

Recent progress in three-dimensional (3D) cell culture systems has attracted much attention in the fields of basic life science and drug development. Newly established methods include 3D co-culture, spheroid culture, and organoid culture; these methods enable more human tissue-like culture and have largely replaced traditional two-dimensional (2D) monolayer culture. By combining 3D culture methods with high-content imaging analysis, it is possible to obtain diverse and convincing data even during initial screening (which requires rapid and easy operating procedures). Until recently, 3D culture methods were considered expensive, time-consuming, complex, and unstable. However, by exploiting the self-assembling nature of cells and adding several technical improvements, we have developed several phenotypic screenings aimed at discovering anticancer compounds.

本文言語English
ページ(範囲)660-666
ページ数7
ジャーナルJournal of Antibiotics
74
10
DOI
出版ステータスPublished - 2021 10

ASJC Scopus subject areas

  • 薬理学
  • 創薬

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