Phorbol ester enhances choline uptake in cultured porcine brain endothelial cells

Takeshi Suzuki, Chiaki Ikeda, Takeshi Fujii, Kazuko Fujimoto, Ikuo Morita, Sei Itsu Murota, Koichiro Kawashima

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Choline uptake in cultured porcine endothelial cells isolated from cerebral microvessels was investigated. A large part of the choline that was taken up was incorporated into phospholipids within 24 h. Phorbol 12-myristate 13-acetate (PMA, 10-7 M), a protein kinase C activator, enhanced choline uptake and acetylcholine (ACh) synthesis in endothelial cells. The PMA-induced enhancement of choline uptake was neither affected by calphostin C (10-7 M), an inhibitor of protein kinase C, nor hemicholinium 3 (10-5 M) treatment. These results suggest that brain endothelial cells have a choline uptake system, which is not identical to the high-affinity choline uptake system located in cholinergic neurons, and that the PMA-induced enhancement of choline uptake is independent of protein kinase C activation. Some of the choline taken up from the extracellular space is used for acetylcholine synthesis.

本文言語English
ページ(範囲)49-54
ページ数6
ジャーナルBiogenic Amines
12
1
出版ステータスPublished - 1996 1月 1

ASJC Scopus subject areas

  • 神経科学(全般)
  • 薬理学

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