Photoacoustic imaging of intravenously injected photosensitizer in rat burn models for efficient antibacterial photodynamic therapy

Yasuyuki Tsunoi, Shunichi Sato, Hiroshi Ashida, Mitsuhiro Terakawa

    研究成果: Conference contribution

    2 被引用数 (Scopus)

    抄録

    For efficient photodynamic treatment of wound infection, a photosensitizer must be distributed in the whole infected tissue region. To ensure this, depth profiling of a photosensitizer is necessary in vivo. In this study, we applied photoacoustic (PA) imaging to visualize the depth profile of an intravenously injected photosensitizer in rat burn models. In burned tissue, pharmacokinetics is complicated; vascular occlusion takes place in the injured tissue, while vascular permeability increases due to thermal invasion. In this study, we first used Evans Blue (EB) as a test drug to examine the feasibility of photosensitizer dosimetry based on PA imaging. On the basis of the results, an actual photosensitizer, talaporfin sodium was used. An EB solution was intravenously injected into a rat deep dermal burn model. PA imaging was performed on the wound with 532 nm and 610 nm nanosecond light pulses for visualizing vasculatures (blood) and EB, respectively. Two hours after injection, the distribution of EB-originated signal spatially coincided well with that of blood-originated signal measured after injury, indicating that EB molecules leaked out from the blood vessels due to increased permeability. Afterwards, the distribution of EB signal was broadened in the depth direction due to diffusion. At 12 hours after injection, clear EB signals were observed even in the zone of stasis, demonstrating that the leaked EB molecules were delivered to the injured tissue layer. The level and time course of talaporfin sodium-originated signals were different compared with those of EB-originated signals, showing animal-dependent and/or drug-dependent permeabilization and diffusion in the tissue. Thus, photosensitizer dosimetry should be needed before every treatment to achieve desirable outcome of photodynamic treatment, for which PA imaging can be concluded to be valid and useful.

    本文言語English
    ホスト出版物のタイトルOptical Methods for Tumor Treatment and Detection
    ホスト出版物のサブタイトルMechanisms and Techniques in Photodynamic Therapy XXI
    DOI
    出版ステータスPublished - 2012 4 9
    イベントOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXI - San Francisco, CA, United States
    継続期間: 2012 1 212012 1 22

    出版物シリーズ

    名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
    8210
    ISSN(印刷版)1605-7422

    Other

    OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXI
    CountryUnited States
    CitySan Francisco, CA
    Period12/1/2112/1/22

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Biomaterials
    • Atomic and Molecular Physics, and Optics
    • Radiology Nuclear Medicine and imaging

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