Physical distance between the site of type II DNA binding to the membrane and oriC on the Bacillus subtilis 168 chromosome

M. Itaya, J. J. Laffan, N. Sueoka

研究成果: Article査読

16 被引用数 (Scopus)

抄録

The precise physical locations of the oriC region and the region for type II DNA binding to the membrane on the Bacillus subtilis 168 chromosome were determined. The DNA regions were physically mapped by creating new restriction sites (NotI and SfiI) within these regions. The physical distance between oriC and the type II DNA-binding region was verified with the creation of a novel sequence cleaved by endonuclease I-SceI in each of the above regions. Complete removal of the defined type II membrane-binding region produced no noticeable phenotype.

本文言語English
ページ(範囲)5466-5470
ページ数5
ジャーナルJournal of Bacteriology
174
16
DOI
出版ステータスPublished - 1992

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学

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