Physical mapping of multiple homologous genes in the Bacillus subtilis 168 chromosome: identification of ten ribosomal RNA operon loci.

Mitsuhiro Itaya

研究成果: Article

6 引用 (Scopus)

抄録

Ten ribosomal RNA operons (rrn) of the Bacillus subtilis 168 strain were precisely located in the complete physical map, i.e., the NotI and SfiI restriction map [M. Itaya and Tanaka, J. Mol. Biol., 220, 631-648 (1991)]. Mapping was done by identification of the NotI and the SfiI fragments to which the rrnO operon hybridized. This protocol proved that homologous multiple genes dispersed on the chromosome can be physically mapped by using a single membrane filter. A previously unidentified deletion formation was shown to be that caused by homologous recombination between rrnJ-W.

元の言語English
ページ(範囲)1611-1614
ページ数4
ジャーナルBioscience, biotechnology, and biochemistry
57
発行部数9
出版物ステータスPublished - 1993 9
外部発表Yes

Fingerprint

Ribosomal RNA
physical chromosome mapping
Bacilli
operon
Operon
Chromosomes
Bacillus subtilis
Genes
ribosomal RNA
chromosomes
loci
Homologous Recombination
homologous recombination
genes
Membranes

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology

これを引用

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