Physical mapping of multiple homologous genes in the bacillus subtilis 168 chromosome: identification of ten ribosomal rna operon loci

Mitsuhiro Itaya

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Ten ribosomal RNA operons (rrn) of the Bacillus subtilis 168 strain were precisely located in the complete physical map, i.e., the NotI and SfiI restriction map [M. Itaya and T. Tanaka, J. Mol. Biol., 220, 631–648 (1991)]. Mapping was done by identification of the NotI and the SfiI fragments to which the rrnO operon hybridized. This protocol proved that homologous multiple genes dispersed on the chromosome can be physically mapped by using a single membrane filter. A previously unidentified deletion formation was shown to be that caused by homologous recombination between rrnJ-W.

本文言語English
ページ(範囲)1611-1614
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
57
9
DOI
出版ステータスPublished - 1993 1月
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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