TY - JOUR
T1 - Physical mapping of multiple homologous genes in the bacillus subtilis 168 chromosome
T2 - identification of ten ribosomal rna operon loci
AU - Itaya, Mitsuhiro
PY - 1993/1
Y1 - 1993/1
N2 - Ten ribosomal RNA operons (rrn) of the Bacillus subtilis 168 strain were precisely located in the complete physical map, i.e., the NotI and SfiI restriction map [M. Itaya and T. Tanaka, J. Mol. Biol., 220, 631–648 (1991)]. Mapping was done by identification of the NotI and the SfiI fragments to which the rrnO operon hybridized. This protocol proved that homologous multiple genes dispersed on the chromosome can be physically mapped by using a single membrane filter. A previously unidentified deletion formation was shown to be that caused by homologous recombination between rrnJ-W.
AB - Ten ribosomal RNA operons (rrn) of the Bacillus subtilis 168 strain were precisely located in the complete physical map, i.e., the NotI and SfiI restriction map [M. Itaya and T. Tanaka, J. Mol. Biol., 220, 631–648 (1991)]. Mapping was done by identification of the NotI and the SfiI fragments to which the rrnO operon hybridized. This protocol proved that homologous multiple genes dispersed on the chromosome can be physically mapped by using a single membrane filter. A previously unidentified deletion formation was shown to be that caused by homologous recombination between rrnJ-W.
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U2 - 10.1271/bbb.57.1611
DO - 10.1271/bbb.57.1611
M3 - Article
C2 - 7693070
AN - SCOPUS:0027661250
VL - 57
SP - 1611
EP - 1614
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 9
ER -