Physical mapping of multiple homologous genes in the bacillus subtilis 168 chromosome: identification of ten ribosomal rna operon loci

Mitsuhiro Itaya

研究成果: Article

6 引用 (Scopus)

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Ten ribosomal RNA operons (rrn) of the Bacillus subtilis 168 strain were precisely located in the complete physical map, i.e., the NotI and SfiI restriction map [M. Itaya and T. Tanaka, J. Mol. Biol., 220, 631–648 (1991)]. Mapping was done by identification of the NotI and the SfiI fragments to which the rrnO operon hybridized. This protocol proved that homologous multiple genes dispersed on the chromosome can be physically mapped by using a single membrane filter. A previously unidentified deletion formation was shown to be that caused by homologous recombination between rrnJ-W.

元の言語English
ページ(範囲)1611-1614
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
57
発行部数9
DOI
出版物ステータスPublished - 1993 1

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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