Physiologic shear stress suppresses endothelin-converting enzyme-1 expression in vascular endothelial cells

Ken Masatsugu, Hiroshi Itoh, Tae Hwa Chun, Yoshihiro Ogawa, Naoshita Tamura, Jun Yamashita, Kentaro Doi, Mayumi Inoue, Yasutomo Fukunaga, Naoki Sawada, Takatoshi Saito, Risa Korenaga, Joji Ando, Kazuwa Nakao

研究成果: Article査読

29 被引用数 (Scopus)

抄録

Shear stress dilates blood vessels and exerts an antiproliferative effect on vascular walls. These effects are ascribed to shear stress-induced, endothelium-derived vasoactive substances. Endothelin-converting enzymes (ECEs), the enzymes that convert big endothelin-1 (ET-1) to ET-1, have recently been isolated and the corresponding proteins have been termed ECE-1 and ECE-2. Furthermore, two isoforms of human ECE-1 have been demonstrated and termed ECE-1α and ECE-1β. In this study, to elucidate the role of ECE-1 under shear stress we examined the effect of physiologic shear stress on the mRNA expression of ECE-1 and ET-1 in cultured bovine carotid artery endothelial cells (BAECs) and human umbilical veins (HUVECs), and also ECE-1α mRNA expression in HUVECs. ECE-1 mRNA expression was significantly downregulated by shear stress in 24 h, both in BAECs and HUVECs, in a shear stress intensity-dependent manner. The expression of ECE-1α mRNA was also attenuated by shear stress in HUVECs. ET-1 mRNA expression showed a concordant decrease with ECE-1 mRNA expression. These results suggest that shear stress-induced gene regulation of ET-1 and ECE-1 mRNA expression can contribute to the decrease of ET-1 peptide level by shear stress.

本文言語English
ページ(範囲)S42-S45
ジャーナルJournal of Cardiovascular Pharmacology
31
SUPPL. 1
DOI
出版ステータスPublished - 1998
外部発表はい

ASJC Scopus subject areas

  • 薬理学
  • 循環器および心血管医学

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