Physiological role of L-type Ca2+ channels in marginal cells in the stria vascularis of guinea pigs

Takaki Inui, Yoshiaki Mori, Masahito Watanabe, Atsuko Takamaki, Junko Yamaji, Yoshiro Sohma, Ryotaro Yoshida, Hiroshi Takenaka, Takahiro Kubota

研究成果: Article

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Using immunohistochemical and electrophysiological methods, we investigated the role of L-type Ca2+ channels in the regulation of the endocochlear potential (EP) of the endolymphatic surface cells (ESC) of the guinea pig stria vascularis. The following findings were made: (1) Administration of 30 μg/ml nifedipine via a vertebral artery significantly suppressed the transient asphyxia-induced decrease in the EP (TAID) and the transient asphyxia-induced increase in the Ca2+, referred to as TAIICa, concentration in the endolymph ([Ca]e).(2) The endolymphatic administration of 1 μg/ml nifedipine significantly inhibited the TAID as well as the TAIICa. The endolymphatic administration of nifedipine (0.001-10 μg/ml) inhibited the TAID in a dose-dependent manner. (3) The endolymphatic administration of (+)-Bay K8644, an L-type Ca2+ channel closer, significantly inhibited the TAID, whereas (-)-Bay K8644, an L-type Ca 2+ channel opener, caused a large decrease in the EP from ∼+75 mV to ∼+20 mV at 10 min after the endolymphatic administration. (4) By means of immunohistochemistry, a positive staining reaction with L-type Ca 2+ channels was detected in the marginal cells of the stria vascularis. (5) Under the high [Ca]e condition, we examined the mechanism of the TAIICa and hypothesized that the TAIICa might have been caused by the decrease in the EP through a shunt pathway in the ESC. (6) The administration of nifedipine to the endolymph significantly inhibited the Ba2+-induced decrease in the EP. These findings support the view that L-type Ca2+ channels in the marginal cells regulate the EP, but not directly the TAIICa.

元の言語English
ページ(範囲)287-298
ページ数12
ジャーナルJournal of Physiological Sciences
57
発行部数5
DOI
出版物ステータスPublished - 2007 10 1

ASJC Scopus subject areas

  • Physiology

フィンガープリント Physiological role of L-type Ca<sup>2+</sup> channels in marginal cells in the stria vascularis of guinea pigs' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Inui, T., Mori, Y., Watanabe, M., Takamaki, A., Yamaji, J., Sohma, Y., Yoshida, R., Takenaka, H., & Kubota, T. (2007). Physiological role of L-type Ca2+ channels in marginal cells in the stria vascularis of guinea pigs. Journal of Physiological Sciences, 57(5), 287-298. https://doi.org/10.2170/physiolsci.RP006807