Pimet, the Drosophila homolog of HEN1, mediates 2′-O-methylation of Piwi-interacting RNAs at their 3′ ends

Kuniaki Saito, Yuriko Sakaguchi, Takeo Suzuki, Tsutomu Suzuki, Haruhiko Siomi, Mikiko C. Siomi

研究成果: Article査読

317 被引用数 (Scopus)

抄録

Piwi-interacting RNAs (piRNAs) consist of a germline-specific group of small RNAs derived from distinct intergenic loci in the genome. piRNAs function in silencing selfish transposable elements through binding with the PIWI subfamily proteins of Argonautes. Here we show that piRNAs in Drosophila are 2′-O-methylated at their 3′ ends. Loss of Pimet (piRNA methyltransferase), the Drosophila homolog of Arabidopsis HEN1 methyltransferase for microRNAs (miRNAs), results in loss of 2′-O-methylation of fly piRNAs. Recombinant Pimet shows single-stranded small RNA methylation activity in vitro and interacts with the PIWI proteins within Pimet mutant ovary. These results show that Pimet mediates piRNA 2′-O-methylation in Drosophila.

本文言語English
ページ(範囲)1603-1608
ページ数6
ジャーナルGenes and Development
21
13
DOI
出版ステータスPublished - 2007 7 1
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 発生生物学

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