Pirfenidone induces intercellular adhesion molecule-1 (ICAM-1) down- regulation on cultured human synovial fibroblasts

M. Kaneko, H. Inoue, R. Nakazawa, N. Azuma, M. Suzuki, S. Yamauchi, S. B. Margolin, K. Tsubota, I. Saito

研究成果: Article査読

75 被引用数 (Scopus)

抄録

Pirfenidone has been shown to modify some cytokine regulatory actions and inhibit fibroblast biochemical reactions resulting in inhibition of proliferation and collagen matrix synthesis by fibroblast. We have investigated the effect of pirfenidone on the expression of cell adhesion molecules. The synovial fibroblasts were treated with IL-1α in the presence or absence of pirfenidone (range 0-1000 μM), and assayed for the expression of adhesion molecules such as ICAM-1 and endothelial-leucocyte adhesion molecule-1 (E-selectin) by cell ELISA. Pirfenidone significantly down- regulated the expression of ICAM-1 on cultured synovial fibroblasts in a dose-dependent manner. In contrast, expression of E-selectin was not affected. Furthermore, we examined whether pirfenidone affects the cellular binding between cultured lymphocytes and IL-1α-stimulated synovial fibroblasts by in vitro binding assay and found their mutual binding was significantly suppressed in a dose-dependent manner by pirfenidone. It is speculated that down-regulation of ICAM-1 might be one of the novel mechanisms of action of pirfenidone. These data indicate a novel mechanism of action for pirfenidone to reduce the activation of synovial fibroblasts.

本文言語English
ページ(範囲)72-76
ページ数5
ジャーナルClinical and Experimental Immunology
113
1
DOI
出版ステータスPublished - 1998
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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