PiRNAs derived from ancient viral processed pseudogenes as transgenerational sequence-specific immune memory in mammals

Nicholas F. Parrish, Kan Fujino, Yusuke Shiromoto, Yuka W. Iwasaki, Hongseok Ha, Jinchuan Xing, Akiko Makino, Satomi Kuramochi-Miyagawa, Toru Nakano, Haruhiko Siomi, Tomoyuki Honda, Keizo Tomonaga

研究成果: Article

33 引用 (Scopus)

抜粋

Endogenous bornavirus-like nucleoprotein elements (EBLNs) are sequences within vertebrate genomes derived from reverse transcription and integration of ancient bornaviral nucleoprotein mRNA via the host retrotransposon machinery. While species with EBLNs appear relatively resistant to bornaviral disease, the nature of this association is unclear. We hypothesized that EBLNs could give rise to antiviral interfering RNA in the form of PIWI-interacting RNAs (piRNAs), a class of small RNA known to silence transposons but not exogenous viruses. We found that in both rodents and primates, which acquired their EBLNs independently some 25-40 million years ago, EBLNs are present within piRNA-generating regions of the genome far more often than expected by chance alone (ℙ = 8 × 10-3-6 × 10-8). Three of the seven human EBLNs fall within annotated piRNA clusters and two marmoset EBLNs give rise to bona fide piRNAs. In both rats and mice, at least two of the five EBLNs give rise to abundant piRNAs in the male gonad. While no EBLNs are syntenic between rodent and primate, some of the piRNA clusters containing EBLNs are; thus we deduce that EBLNs were integrated into existing piRNA clusters. All true piRNAs derived from EBLNs are antisense relative to the proposed ancient bornaviral nucleoprotein mRNA. These observations are consistent with a role for EBLN-derived piRNA-like RNAs in interfering with ancient bornaviral infection. They raise the hypothesis that retrotransposon-dependent virus-to-host gene flow could engender RNA-mediated, sequence-specific antiviral immune memory in metazoans analogous to the CRISPR/Cas system in prokaryotes.

元の言語English
ページ(範囲)1691-1703
ページ数13
ジャーナルRNA
21
発行部数10
DOI
出版物ステータスPublished - 2015 10 1

ASJC Scopus subject areas

  • Molecular Biology

フィンガープリント PiRNAs derived from ancient viral processed pseudogenes as transgenerational sequence-specific immune memory in mammals' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Parrish, N. F., Fujino, K., Shiromoto, Y., Iwasaki, Y. W., Ha, H., Xing, J., Makino, A., Kuramochi-Miyagawa, S., Nakano, T., Siomi, H., Honda, T., & Tomonaga, K. (2015). PiRNAs derived from ancient viral processed pseudogenes as transgenerational sequence-specific immune memory in mammals. RNA, 21(10), 1691-1703. https://doi.org/10.1261/rna.052092.115