Piwi Nuclear Localization and Its Regulatory Mechanism in Drosophila Ovarian Somatic Cells

Ryu Yashiro, Yukiko Murota, Kazumichi M. Nishida, Haruna Yamashiro, Kaede Fujii, Asuka Ogai, Soichiro Yamanaka, Lumi Negishi, Haruhiko Siomi, Mikiko C. Siomi

研究成果: Article

16 引用 (Scopus)

抜粋

In Drosophila ovarian somatic cells (OSCs), Piwi represses transposons transcriptionally to maintain genome integrity. Piwi nuclear localization requires the N terminus and PIWI-interacting RNA (piRNA) loading of Piwi. However, the underlying mechanism remains unknown. Here, we show that Importinα (Impα) plays a pivotal role in Piwi nuclear localization and that Piwi has a bipartite nuclear localization signal (NLS). Impα2 and Impα3 are highly expressed in OSCs, whereas Impα1 is the least expressed. Loss of Impα2 or Impα3 forces Piwi to be cytoplasmic, which is rectified by overexpression of any Impα members. Extension of Piwi-NLS with an additional Piwi-NLS leads Piwi to be imported to the nucleus in a piRNA-independent manner, whereas replacement of Piwi-NLS with SV40-NLS fails. Limited proteolysis analysis suggests that piRNA loading onto Piwi triggers conformational change, exposing the N terminus to the environment. These results suggest that Piwi autoregulates its nuclear localization by exposing the NLS to Impα upon piRNA loading. Piwi represses transposons transcriptionally to maintain genome integrity. Yashiro et al. show that Importinα plays a pivotal role in Piwi nuclear localization and that Piwi has a bipartite nuclear localization signal at the N terminus. Piwi autoregulates its nuclear localization by exposing the nuclear localization signal to Impα upon piRNA loading.

元の言語English
ページ(範囲)3647-3657
ページ数11
ジャーナルCell Reports
23
発行部数12
DOI
出版物ステータスPublished - 2018 6 19

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Yashiro, R., Murota, Y., Nishida, K. M., Yamashiro, H., Fujii, K., Ogai, A., Yamanaka, S., Negishi, L., Siomi, H., & Siomi, M. C. (2018). Piwi Nuclear Localization and Its Regulatory Mechanism in Drosophila Ovarian Somatic Cells. Cell Reports, 23(12), 3647-3657. https://doi.org/10.1016/j.celrep.2018.05.051