PM2.5-induced airway inflammation and hyperresponsiveness in NC/Nga mice

Keiki Ogino, Kenjiro Nagaoka, Tomoaki Okuda, Akira Oka, Masayuki Kubo, Eri Eguchi, Yoshihisa Fujikura

研究成果: Article査読

32 被引用数 (Scopus)

抄録

The allergic inflammatory effects of particulate matter (PM) 2.5, collected with the cyclone system in Yokohama city in Japan, were investigated in NC/Nga mice, which are hypersensitive to mite allergens. PM2.5 with alum was injected intraperitoneally for sensitization. Five days later, 200 μg of PM2.5 in 25 μL of saline was administered to mice intranasally five times for further sensitization. On the 11th day, PM2.5 was administered as a challenge. On the 12th day, mice were examined for airway hyperresponsiveness (AHR), the bronchoalveolar lavage fluid (BALF) cell count, mRNA expression of Th1, Th2 cytokines, and metallothioneins in lung tissue, and histopathology. PM2.5 increased AHR, total cell numbers including eosinophils in BALF, and mRNA levels of IL-5, IL-22, eotaxin, eotaxin 2, and metallothionein 3. In PM2.5-induced lungs, inflammation was observed around the bronchus. These results demonstrate that PM2.5 alone, collected with the cyclone system in Yokohama city in Japan, induces asthma-like airway inflammation.

本文言語English
ページ(範囲)1047-1054
ページ数8
ジャーナルEnvironmental Toxicology
32
3
DOI
出版ステータスPublished - 2017 3 1

ASJC Scopus subject areas

  • 毒物学
  • 管理、モニタリング、政策と法律
  • 健康、毒物学および変異誘発

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