Polysaccharide-Coated Immunoliposomes Bearing Anti-CEA Fab' Fragment and Their Internalization by CEA-Producing Tumor Cells

Toshinori Sato, Junzo Sunamoto, Nobuko Ishii, Toshihiko Koji

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Immunoliposomes, bearing Fab' fragments of anti-carcinoembryonic antigen (anti-CEA) monoclonal antibody, have been prepared according to a new method developed in our laboratory. First, egg phosphatidylcholine liposomes were coated with pullulan derivatives. Then, SH-bearing Fab' fragments of anti-CEA were conjugated to the pullulan derivatives attached to the liposomal surface. These immunoliposomes showed specific binding to CEA-producing cells, BM314, which was approximately 20-fold greater than to CEA-non- producing cells, RPMI #4788. In addition, these immunoliposomes were endo cytosed by colon cancer cells, which was confirmed by fluorescence microscopy using liposomes labelled by a hydrophobic fluorescent probe, terbium trisace tylacetonate.

本文言語English
ページ(範囲)195-204
ページ数10
ジャーナルJournal of Bioactive and Compatible Polymers
3
3
DOI
出版ステータスPublished - 1988 7
外部発表はい

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 生体材料
  • ポリマーおよびプラスチック
  • 材料化学

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