Porcupine Controls Hippocampal AMPAR Levels, Composition, and Synaptic Transmission

Nadine Erlenhardt, Hong Yu, Kavitha Abiraman, Tokiwa Yamasaki, Jacques I. Wadiche, Susumu Tomita, David S. Bredt

研究成果: Article査読

33 被引用数 (Scopus)

抄録

AMPA receptor (AMPAR) complexes contain auxiliary subunits that modulate receptor trafficking and gating. In addition to the transmembrane AMPAR regulatory proteins (TARPs) and cornichons (CNIH-2/3), recent proteomic studies identified a diverse array of additional AMPAR-associated transmembrane and secreted partners. We systematically surveyed these and found that PORCN and ABHD6 increase GluA1 levels in transfected cells. Knockdown of PORCN in rat hippocampal neurons, which express it in high amounts, selectively reduces levels of all tested AMPAR complex components. Regulation of AMPARs is independent of PORCN's membrane-associated O-acyl transferase activity. PORCN knockdown in hippocampal neurons decreases AMPAR currents and accelerates desensitization and leads to depletion of TARP γ-from AMPAR complexes.

本文言語English
ページ(範囲)782-794
ページ数13
ジャーナルCell Reports
14
4
DOI
出版ステータスPublished - 2016 2月 2
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

フィンガープリント

「Porcupine Controls Hippocampal AMPAR Levels, Composition, and Synaptic Transmission」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル