POSSIBLE ROLE OF METALLOTHIONEIN IN THE CELLULAR DEFENSE MECHANISM AGAINST UVB IRRADIATION IN NEONATAL HUMAN SKIN FIBROBLASTS

Shizuko Kobayashi, Yumiko Hirota, Junko Sayato‐Suzuki, Makoto Takehana, Hisao Nishimura, Noriko Nishimura, Chiharu Tohyama

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Abstract: The role of metallothionein (MT) in protecting skin cells against UVB irradiation was investigated. Fibroblast strains from normal adult (HS‐K) and neonatal (NB1RGB) human skins as well as keratinocyte strains from human skin (SV40‐HSK) and newborn Balb/c mouse skin (Pam 212) were exposed to UVB irradiation. The sensitivity of HS‐K and NB1RGB cells to UVB irradiation was similar; those of SV40‐HSK and Pam 212 cells were two‐ and six‐fold as sensitive to UVB irradiation as HS‐K cells, respectively. The HS‐K cells contained the greatest cellular reduced form of glutathione (GSH) levels compared to the three other skin cells: the levels were 13‐, 7‐ and 6‐fold of those in NB1RGB, SV40‐HSK and Pam 212 cells, respectively. These results indicated that the sensitivity of skin cells to UVB irradiation was not always associated with their endogenous GSH levels. In particular, despite the fact that NB1RGB cells contained a relatively small amount of GSH, they were less sensitive to UVB irradiation. NB1RGB cells contained 4–30 times more MT than those in other skin cells examined. The sulfhydryl residues of MT molecules in the NB1RGB cells were estimated to be mostly unoccupied by metals, suggesting they act in a similar way to those of GSH. Moreover, NB1RGB cells in which the MT content was elevated by dexamethasone (1 μM) or Zn2+ (7 μg/mL) treatment were more resistant to UVB irradiation than nontreated ones. These results suggest that, at least in neonatal human skin fibroblasts, MT may play a role in protection against UVB irradiation.

本文言語English
ページ(範囲)650-656
ページ数7
ジャーナルPhotochemistry and Photobiology
59
6
DOI
出版ステータスPublished - 1994 6月

ASJC Scopus subject areas

  • 生化学
  • 物理化学および理論化学

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