Postischemic hypothermia attenuates apoptotic cell death in transient focal ischemia in rats.

J. Inamasu, S. Suga, S. Sato, T. Horiguchi, K. Akaji, K. Mayanagi, T. Kawase

研究成果: Article

36 引用 (Scopus)

抜粋

Hypothermia confers potent neuroprotection against ischemic injury. Attenuation of apoptosis by hypothermia can be one of the responsible mechanisms. In this study, in situ DNA nick-end labeling (TUNEL) and immunostaining of Bax protein were performed to evaluate the effect of postischemic hypothermia on apoptotic cell death, employing rodent transient focal ischemia. Animals received 1 hour of transient focal ischemia. Brain temperature was maintained at 37.5 +/- 0.5 degrees C during ischemia. Immediately after reperfusion, animals were assigned to either a normothermic or hypothermic group. In hypothermia, animals were cooled and brain temperature was lowered to 34.5 +/- 1.0 degrees C. Prolonged hypothermia was maintained for 16 hours and animals rewarmed. In both groups, TUNEL and immunostaining of Bax was performed. In normothermia, the number of TUNEL positive cells reached the peak at 2 days after ischemia and decreased gradually. In hypothermia, the peak was shifted to 3 days after ischemia. The number of TUNEL positive cells in hypothermia was persistently below that of normothermia. Similarly, in hypothermia, immunostaining of Bax showed attenuated immunoreactivity compared with that in normothermia. In conclusion, postischemic hypothermia reduced both the number of TUNEL positive cells and immunoreactivity of Bax, which may be one of the responsible mechanisms with which hypothermia exerts neuroprotection.

元の言語English
ページ(範囲)525-527
ページ数3
ジャーナルActa neurochirurgica. Supplement
76
出版物ステータスPublished - 2000

    フィンガープリント

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

これを引用

Inamasu, J., Suga, S., Sato, S., Horiguchi, T., Akaji, K., Mayanagi, K., & Kawase, T. (2000). Postischemic hypothermia attenuates apoptotic cell death in transient focal ischemia in rats. Acta neurochirurgica. Supplement, 76, 525-527.