Preferential transcription of HTLV-I LTR in cell-free extracts of human T cells producing HTLV-I viral proteins

Norisada Matsunami, Haruhiko Siomi, Masakazu Hatanaka, Yoshio Yaoita, Tasuku Honjo

研究成果: Article査読

1 被引用数 (Scopus)

抄録

The promoters of the adenovlrus 2 major late gene, the mouse β-globin gene, the mouse lmmunoglobulin VH gene and the LTR of the human T-lympho-tropic retrovirus type I were tested for their transcription activities in cell-free extracts of four cell lines; HeLa, CESS (Epstein-Barr virus-transformed human B cell line), MT-1 (HTLV-I-infected human T cell line without viral protein synthesis), and MT-2 (HTLV-I-infected hitman T cell line producing viral proteins). LTR was preferentially transcribed in the extracts of MT-2 although the other three genes were transcribed with relatively constant efficiencies in different extracts. The results agree well with the previous in vivo studies on the promoter activity of HTLV-I LTR. Mixing of HeLa and MT-2 extracts revealed the presence of a LTR-specific stimulating activity in MT-2 extracts.

本文言語English
ページ(範囲)4779-4786
ページ数8
ジャーナルNucleic acids research
14
12
DOI
出版ステータスPublished - 1986 6 25
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

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