TY - JOUR
T1 - Preoperative Aqueous Cytokine Levels Are Associated With a Rapid Reduction in Endothelial Cells After Penetrating Keratoplasty
AU - Yagi-Yaguchi, Yukari
AU - Yamaguchi, Takefumi
AU - Higa, Kazunari
AU - Suzuki, Terumasa
AU - Yazu, Hiroyuki
AU - Aketa, Naohiko
AU - Satake, Yoshiyuki
AU - Tsubota, Kazuo
AU - Shimazaki, Jun
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/9
Y1 - 2017/9
N2 - Purpose To evaluate the influence of preoperative inflammatory cytokine levels in the aqueous humor (AqH) on the endothelial cell density (ECD) after penetrating keratoplasty (PKP). Design Prospective, interventional, consecutive case series. Methods This study includes 70 consecutive patients (mean age 73.7 ± 10.6 years) who underwent PKP (37 eyes) or cataract surgery (controls, 33 eyes). A total of 70 AqH samples were collected at the beginning of each surgery. The levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, interferon [IFN]-α, IFN-γ, monocyte chemotactic protein [MCP]-1, E-selectin and P-selectin) in AqH were measured by multiplex bead immunoassay. The subjects who underwent PKP were classified into 2 groups: ECD ≥ 1200 cells/mm2 at 6 months (24 eyes), and ECD < 1200 cells/mm2 at 6 months (13 eyes). Results The ECD at 3 months significantly correlated with the preoperative levels of IL-10 (r = −0.428, P =.02) and IFN-γ (r = −0.412, P =.029). The ECD at 6 months significantly correlated with the preoperative levels of IL-10 (r = −0.399, P =.024), MCP-1 (r = −0.444, P =.011), and IFN-γ (r = −0.474, P =.006). The preoperative levels of IL-6, IL-10, MCP-1, IFN-γ, and P-selectin in AqH were significantly higher in eyes with ECD < 1200 cells/mm2 compared with those with ECD ≥ 1200 cells/mm2 at 6 months (P <.05). Conclusions Higher preoperative levels of IL-10, MCP-1, and IFN-γ in the AqH were associated with low ECD after PKP.
AB - Purpose To evaluate the influence of preoperative inflammatory cytokine levels in the aqueous humor (AqH) on the endothelial cell density (ECD) after penetrating keratoplasty (PKP). Design Prospective, interventional, consecutive case series. Methods This study includes 70 consecutive patients (mean age 73.7 ± 10.6 years) who underwent PKP (37 eyes) or cataract surgery (controls, 33 eyes). A total of 70 AqH samples were collected at the beginning of each surgery. The levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, interferon [IFN]-α, IFN-γ, monocyte chemotactic protein [MCP]-1, E-selectin and P-selectin) in AqH were measured by multiplex bead immunoassay. The subjects who underwent PKP were classified into 2 groups: ECD ≥ 1200 cells/mm2 at 6 months (24 eyes), and ECD < 1200 cells/mm2 at 6 months (13 eyes). Results The ECD at 3 months significantly correlated with the preoperative levels of IL-10 (r = −0.428, P =.02) and IFN-γ (r = −0.412, P =.029). The ECD at 6 months significantly correlated with the preoperative levels of IL-10 (r = −0.399, P =.024), MCP-1 (r = −0.444, P =.011), and IFN-γ (r = −0.474, P =.006). The preoperative levels of IL-6, IL-10, MCP-1, IFN-γ, and P-selectin in AqH were significantly higher in eyes with ECD < 1200 cells/mm2 compared with those with ECD ≥ 1200 cells/mm2 at 6 months (P <.05). Conclusions Higher preoperative levels of IL-10, MCP-1, and IFN-γ in the AqH were associated with low ECD after PKP.
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U2 - 10.1016/j.ajo.2017.07.005
DO - 10.1016/j.ajo.2017.07.005
M3 - Article
C2 - 28734818
AN - SCOPUS:85026745982
VL - 181
SP - 166
EP - 173
JO - American Journal of Ophthalmology
JF - American Journal of Ophthalmology
SN - 0002-9394
ER -