Preparation and biological application of fluorescent poly(methyl methacrylate) Beads Hybridized with Y3Al5O 12:Ce3+ nanophosphor

Ikumi Kusayama, Ryo Asakura, Daisuke Saito, Tetsuhiko Isobe, Kiyoshi Kurokawa, Hideki Aizawa, Tomohiro Takagi, Yosuke Hirayama, Michio Ohkubo

研究成果: Conference contribution

抜粋

Positively charged Y3Al5O12Ce3+ nanoparticles and negatively charged poly(sodium 4-styrenesulfonate) are sequentially adsorbed on the surface of negatively charged poly(methyl methacrylate) beads by the layer-by-layer adsorption method to obtain the composite beads, showing green emission under the blue light excitation. These fluorescent composite beads are detected as a dot group by flowcytometry (FCM). Bovine serum albumin (BSA) immomobilized on the composite beads with green emission are mixed with anti-BSA tagged with organic red dye. The antigen-antibody reaction is detected from both green and red emissions by FCM, showing that the fluorescent composite beads can be used for detecting biological reactions.

元の言語English
ホスト出版物のタイトルECS Transactions - Physics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors
ページ69-74
ページ数6
エディション31
出版物ステータスPublished - 2009 11 25
イベントPhysics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors - 214th ECS Meeting - Honolulu, HI, United States
継続期間: 2008 10 122008 10 17

出版物シリーズ

名前ECS Transactions
番号31
16
ISSN(印刷物)1938-5862
ISSN(電子版)1938-6737

Other

OtherPhysics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors - 214th ECS Meeting
United States
Honolulu, HI
期間08/10/1208/10/17

ASJC Scopus subject areas

  • Engineering(all)

フィンガープリント Preparation and biological application of fluorescent poly(methyl methacrylate) Beads Hybridized with Y<sub>3</sub>Al<sub>5</sub>O <sub>12</sub>:Ce<sup>3+</sup> nanophosphor' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Kusayama, I., Asakura, R., Saito, D., Isobe, T., Kurokawa, K., Aizawa, H., Takagi, T., Hirayama, Y., & Ohkubo, M. (2009). Preparation and biological application of fluorescent poly(methyl methacrylate) Beads Hybridized with Y3Al5O 12:Ce3+ nanophosphor. : ECS Transactions - Physics and Chemistry of Luminescent Materials, Including the 4th Symposium on Persistent Phosphors (31 版, pp. 69-74). (ECS Transactions; 巻数 16, 番号 31).