抄録
Hollow nanoparticles (bionanocapsules) were prepared using liposomes as a template for the layerby-layer deposition of biopolymers. We carried out the alternative deposition of poly-L-lysine (PLys) and poly-L-aspartic acid (PAsP) onto a negatively charged liposome, which was prepared from dilauroyl phosphatidic acid (DLPA) and dimyristoylphosphatidylcholine (DMPC). Efficient separation of nanocapsules from unbound polymers was achieved by ultrafiltration. ζ-Potentials of nanocapsules changed between positive and negative charges at each deposition. FE-TEM revealed that the liposome was covered with a thin polymeric layer. A fluorescent probe, l-hydroxypyrene-3,6,8- trisulfonic acid (HPTS), was encapsulated into nanocapsules. The release of HPTS was suppressed by the polymer deposition, and the release rate was tunable by coverage of the first PLys layer and the ratio of DLPA.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 5122-5128 |
| ページ数 | 7 |
| ジャーナル | Macromolecules |
| 巻 | 40 |
| 発行部数 | 14 |
| DOI | |
| 出版物ステータス | Published - 2007 7 10 |
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ASJC Scopus subject areas
- Materials Chemistry
これを引用
Preparation of bionanocapsules by the layer-by-layer deposition of polypeptides onto a liposome. / Fujimoto, Keiji; Toyoda, Tomonori; Fukui, Yuuka.
：: Macromolecules, 巻 40, 番号 14, 10.07.2007, p. 5122-5128.研究成果: Article
}
TY - JOUR
T1 - Preparation of bionanocapsules by the layer-by-layer deposition of polypeptides onto a liposome
AU - Fujimoto, Keiji
AU - Toyoda, Tomonori
AU - Fukui, Yuuka
PY - 2007/7/10
Y1 - 2007/7/10
N2 - Hollow nanoparticles (bionanocapsules) were prepared using liposomes as a template for the layerby-layer deposition of biopolymers. We carried out the alternative deposition of poly-L-lysine (PLys) and poly-L-aspartic acid (PAsP) onto a negatively charged liposome, which was prepared from dilauroyl phosphatidic acid (DLPA) and dimyristoylphosphatidylcholine (DMPC). Efficient separation of nanocapsules from unbound polymers was achieved by ultrafiltration. ζ-Potentials of nanocapsules changed between positive and negative charges at each deposition. FE-TEM revealed that the liposome was covered with a thin polymeric layer. A fluorescent probe, l-hydroxypyrene-3,6,8- trisulfonic acid (HPTS), was encapsulated into nanocapsules. The release of HPTS was suppressed by the polymer deposition, and the release rate was tunable by coverage of the first PLys layer and the ratio of DLPA.
AB - Hollow nanoparticles (bionanocapsules) were prepared using liposomes as a template for the layerby-layer deposition of biopolymers. We carried out the alternative deposition of poly-L-lysine (PLys) and poly-L-aspartic acid (PAsP) onto a negatively charged liposome, which was prepared from dilauroyl phosphatidic acid (DLPA) and dimyristoylphosphatidylcholine (DMPC). Efficient separation of nanocapsules from unbound polymers was achieved by ultrafiltration. ζ-Potentials of nanocapsules changed between positive and negative charges at each deposition. FE-TEM revealed that the liposome was covered with a thin polymeric layer. A fluorescent probe, l-hydroxypyrene-3,6,8- trisulfonic acid (HPTS), was encapsulated into nanocapsules. The release of HPTS was suppressed by the polymer deposition, and the release rate was tunable by coverage of the first PLys layer and the ratio of DLPA.
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UR - http://www.scopus.com/inward/citedby.url?scp=34547425936&partnerID=8YFLogxK
U2 - 10.1021/ma070477w
DO - 10.1021/ma070477w
M3 - Article
AN - SCOPUS:34547425936
VL - 40
SP - 5122
EP - 5128
JO - Macromolecules
JF - Macromolecules
SN - 0024-9297
IS - 14
ER -