Preparation of dimerized polypeptide-carrying microspheres and purification of specific proteins bound to these microspheres

M. Hatakeyama, Y. Ogura, J. Sawada, K. Fujimoto, H. Handa, H. Kawaguchi

研究成果: Article査読

3 被引用数 (Scopus)

抄録

We have developed polypeptide-carrying microspheres for bioaffinity separation. The carrier microspheres were composed of a polystyrene core and a poly(glycidyl methacrylate) surface layer on which ethyleneglycol diglycidyl ether was introduced as a spacer. The epoxy group of the spacer's end was hydrolyzed. The hydroxy end group was then activated by tosyl(p-toluenesulfonyl) chloride. To tosyl-group-carrying microspheres, we immobilized a polypeptide, HT-Lys5-N236, which contains the carboxyl terminal region of transcription factor hGABP β, analyzed the structure of the polypeptide immobilized on the surface of microspheres and used these hybrid microspheres for the purification of N236-binding protein from HeLa cell nuclear extracts directly and from the fractions separated with a phosphocellulose column.

本文言語English
ページ(範囲)41-49
ページ数9
ジャーナルColloids and Surfaces B: Biointerfaces
10
1
DOI
出版ステータスPublished - 1997 10

ASJC Scopus subject areas

  • バイオテクノロジー
  • 表面および界面
  • 物理化学および理論化学
  • コロイド化学および表面化学

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