Preparation of DNA-carrying affinity latex and purification of transcription factors with the latex.

Y. Inomata, T. Wada, H. Handa, Keiji Fujimoto, H. Kawaguchi

研究成果: Article

65 引用 (Scopus)

抄録

We have developed DNA-carrying latex particles for the separation and purification of transcription factors. These particles consist of styrene (St), glycidyl methacrylate (GMA) and divinylbenzene (DVB). It was confirmed that the ethanolamine-treated surface of these particles suffered no nonspecific adsorption of proteins. To the latex particles sequence-specific DNA oligomers were immobilized via covalent coupling. A transcription factor, E4TF3, was efficiently purified to homogeneity using the latext particles. In contrast, the purification using DNA-carrying Sepharose gel yielded poor results. Compared to DNA-carrying Sepharose gel, the latex particles exhibited several times higher efficiency in the purification of E4TF3 from the crude nuclear extract.

元の言語English
ページ(範囲)293-302
ページ数10
ジャーナルJournal of biomaterials science. Polymer edition
5
発行部数4
出版物ステータスPublished - 1994

Fingerprint

Transcription factors
Latex
Latexes
Microspheres
Purification
divinyl benzene
DNA
Transcription Factors
Particles (particulate matter)
Sepharose
Gels
Ethanolamine
Styrene
Ethanolamines
Complex Mixtures
Adsorption
Oligomers
Proteins

ASJC Scopus subject areas

  • Biophysics

これを引用

Preparation of DNA-carrying affinity latex and purification of transcription factors with the latex. / Inomata, Y.; Wada, T.; Handa, H.; Fujimoto, Keiji; Kawaguchi, H.

:: Journal of biomaterials science. Polymer edition, 巻 5, 番号 4, 1994, p. 293-302.

研究成果: Article

@article{1536dcd5a447491fa504da2230effac8,
title = "Preparation of DNA-carrying affinity latex and purification of transcription factors with the latex.",
abstract = "We have developed DNA-carrying latex particles for the separation and purification of transcription factors. These particles consist of styrene (St), glycidyl methacrylate (GMA) and divinylbenzene (DVB). It was confirmed that the ethanolamine-treated surface of these particles suffered no nonspecific adsorption of proteins. To the latex particles sequence-specific DNA oligomers were immobilized via covalent coupling. A transcription factor, E4TF3, was efficiently purified to homogeneity using the latext particles. In contrast, the purification using DNA-carrying Sepharose gel yielded poor results. Compared to DNA-carrying Sepharose gel, the latex particles exhibited several times higher efficiency in the purification of E4TF3 from the crude nuclear extract.",
author = "Y. Inomata and T. Wada and H. Handa and Keiji Fujimoto and H. Kawaguchi",
year = "1994",
language = "English",
volume = "5",
pages = "293--302",
journal = "Journal of Biomaterials Science, Polymer Edition",
issn = "0920-5063",
publisher = "Taylor and Francis Ltd.",
number = "4",

}

TY - JOUR

T1 - Preparation of DNA-carrying affinity latex and purification of transcription factors with the latex.

AU - Inomata, Y.

AU - Wada, T.

AU - Handa, H.

AU - Fujimoto, Keiji

AU - Kawaguchi, H.

PY - 1994

Y1 - 1994

N2 - We have developed DNA-carrying latex particles for the separation and purification of transcription factors. These particles consist of styrene (St), glycidyl methacrylate (GMA) and divinylbenzene (DVB). It was confirmed that the ethanolamine-treated surface of these particles suffered no nonspecific adsorption of proteins. To the latex particles sequence-specific DNA oligomers were immobilized via covalent coupling. A transcription factor, E4TF3, was efficiently purified to homogeneity using the latext particles. In contrast, the purification using DNA-carrying Sepharose gel yielded poor results. Compared to DNA-carrying Sepharose gel, the latex particles exhibited several times higher efficiency in the purification of E4TF3 from the crude nuclear extract.

AB - We have developed DNA-carrying latex particles for the separation and purification of transcription factors. These particles consist of styrene (St), glycidyl methacrylate (GMA) and divinylbenzene (DVB). It was confirmed that the ethanolamine-treated surface of these particles suffered no nonspecific adsorption of proteins. To the latex particles sequence-specific DNA oligomers were immobilized via covalent coupling. A transcription factor, E4TF3, was efficiently purified to homogeneity using the latext particles. In contrast, the purification using DNA-carrying Sepharose gel yielded poor results. Compared to DNA-carrying Sepharose gel, the latex particles exhibited several times higher efficiency in the purification of E4TF3 from the crude nuclear extract.

UR - http://www.scopus.com/inward/record.url?scp=0028188071&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028188071&partnerID=8YFLogxK

M3 - Article

C2 - 8025028

AN - SCOPUS:0028188071

VL - 5

SP - 293

EP - 302

JO - Journal of Biomaterials Science, Polymer Edition

JF - Journal of Biomaterials Science, Polymer Edition

SN - 0920-5063

IS - 4

ER -