Preparation of dna-carrying affinity latex and purification of transcription factors with the latex

Y. Inomata, T. Wada, H. Handa, K. Fujimoto, H. Kawaguchi

研究成果: Article査読

65 被引用数 (Scopus)

抄録

We have developed DNA-carrying latex particles for the separation and purification of transcription factors. These particles consist of styrene (St), glycidyl methacrylate (GMA) and divinylbenzene (DVB). It was confirmed that the ethanolamine-treated surface of these particles suffered no nonspecific adsorption of proteins. To the latex particles sequence-specific DNA oligomers were immobilized via covalent coupling. A transcription factor, E4TF3, was efficiently purified to homogeneity using the latext particles. In contrast, the purification using DNA-carrying Sepharose gel yielded poor results. Compared to DNA-carrying Sepharose gel, the latex particles exhibited several times higher efficiency in the purification of E4TF3 from the crude nuclear extract.

本文言語English
ページ(範囲)293-302
ページ数10
ジャーナルJournal of Biomaterials Science, Polymer Edition
5
4
DOI
出版ステータスPublished - 1994 1 1

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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