Preparation of fluorescent poly(methyl methacrylate) beads hybridized with y₃Al₅O₁₂:Ce³⁺ nanophosphor for biological application

Poly(methyl methacrylate) beads were hybridized with Y₃Al ₅O₁₂:Ce³⁺ nanoparticles through electrostatic interactions using the layer-by-layer adsorption technique to prepare fluorescent beads for biological application. The subsequent sequential adsorption of poly(sodium 4-styrenesulfonate) and poly(allylamine hydrochloride) onto the composite beads prevented the detachment of the Y₃Al ₅O₁₂:Ce³⁺ nanoparticles. The fluorescent beads were observed as tight dot plots by flowcytometry. Bovine serum albumin can be immobilized on the surface of the composite beads and tagged with a red dye through an antigen-antibody reaction. This result indicates that the composite beads are useful for bead assays such as flowcytometry.