Preparation of fluorescent poly(methyl methacrylate) beads hybridized with y3Al5O12:Ce3+ nanophosphor for biological application

Ryo Asakura, Ikumi Kusayama, Daisuke Saito, Tetsuhiko Isobe, Kiyoshi Kurokawa, Yosuke Hirayama, Hideki Aizawa, Tomohiro Takagi, Michio Ohkubo

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Poly(methyl methacrylate) beads were hybridized with Y3Al 5O12:Ce3+ nanoparticles through electrostatic interactions using the layer-by-layer adsorption technique to prepare fluorescent beads for biological application. The subsequent sequential adsorption of poly(sodium 4-styrenesulfonate) and poly(allylamine hydrochloride) onto the composite beads prevented the detachment of the Y3Al 5O12:Ce3+ nanoparticles. The fluorescent beads were observed as tight dot plots by flowcytometry. Bovine serum albumin can be immobilized on the surface of the composite beads and tagged with a red dye through an antigen-antibody reaction. This result indicates that the composite beads are useful for bead assays such as flowcytometry.

本文言語English
ページ(範囲)5193-5195
ページ数3
ジャーナルJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
46
8 A
DOI
出版ステータスPublished - 2007 8 6

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

フィンガープリント 「Preparation of fluorescent poly(methyl methacrylate) beads hybridized with y<sub>3</sub>Al<sub>5</sub>O<sub>12</sub>:Ce<sup>3+</sup> nanophosphor for biological application」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル