Preparation of optically active 4-chlorophenylalanine from its racemate by deracemization technique using transformant Escherichia coli cells

Dai Ichiro Kato, Kenji Miyamoto, Hiromichi Ohta

研究成果: Article査読

13 被引用数 (Scopus)

抄録

We have developed a method for the preparation of L-4-chlorophenylalanine from its racemate with Escherichia coli cells expressing a single foreign gene. L-4-Chlorophenylalanine was obtained in a high optical yield by the inversion of configuration of its d-form via the tandem reactions catalyzed by d-amino acid dehydrogenase (DadA) and branched-chain amino acid aminotransferase (BCAAT). While we constructed a plasmid for BCAAT utilizing the gene from Sinorhizobium meliloti ATCC 51124, the first enzyme DadA was the dadA-gene product from E. coli host cell itself, which was activated by the addition of L-alanine in the growth medium.

本文言語English
ページ(範囲)375-379
ページ数5
ジャーナルBiocatalysis and Biotransformation
23
5
DOI
出版ステータスPublished - 2005 9

ASJC Scopus subject areas

  • バイオテクノロジー
  • 触媒
  • 生化学

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