Preparation Of Peptide-Carrying Microspheres With Bioactivity On Platelets

Yuji Kasuya; Keiji Fujimoto; Haruma Kawaguchi; Masaki Miyamoto; Takeo Juji; Akira Otaka; Susumu Funakoshi; Nobutaka Fujii

doi:10.1163/156856293X00050

Preparation Of Peptide-Carrying Microspheres With Bioactivity On Platelets

Yuji Kasuya, Keiji Fujimoto, Haruma Kawaguchi, Masaki Miyamoto, Takeo Juji, Akira Otaka, Susumu Funakoshi, Nobutaka Fujii

応用化学科

研究成果: Article › 査読

12 被引用数 (Scopus)

抄録

A tetrapeptide RGDS (Arg-Gly-Asp-Ser), which is one of the active sites of cell adhesive proteins, was synthesized according to the solution method and covalently coupled onto monodisperse microspheres. The biospecific activity of the RGDS-carrying microspheres was investigated by measuring the stimulatory effect of the microspheres on platelets. Large and tightly packed platelet aggregation accompanied with drastic ATP release was induced by the RGDS-carrying microspheres. It was confirmed that the specific platelet aggregation was attributed to the activity of the immobilized RGDS by comparison with those induced by other microspheres.

本文言語	English
ページ（範囲）	369-380
ページ数	12
ジャーナル	Journal of Biomaterials Science, Polymer Edition
巻	4
号	4
DOI	https://doi.org/10.1163/156856293X00050
出版ステータス	Published - 1993

ASJC Scopus subject areas

生物理学
バイオエンジニアリング
生体材料
生体医工学

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10.1163/156856293X00050

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abstract = "A tetrapeptide RGDS (Arg-Gly-Asp-Ser), which is one of the active sites of cell adhesive proteins, was synthesized according to the solution method and covalently coupled onto monodisperse microspheres. The biospecific activity of the RGDS-carrying microspheres was investigated by measuring the stimulatory effect of the microspheres on platelets. Large and tightly packed platelet aggregation accompanied with drastic ATP release was induced by the RGDS-carrying microspheres. It was confirmed that the specific platelet aggregation was attributed to the activity of the immobilized RGDS by comparison with those induced by other microspheres.",

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AU - Kasuya, Yuji

AU - Fujimoto, Keiji

AU - Kawaguchi, Haruma

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AU - Juji, Takeo

AU - Otaka, Akira

AU - Funakoshi, Susumu

AU - Fujii, Nobutaka

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AB - A tetrapeptide RGDS (Arg-Gly-Asp-Ser), which is one of the active sites of cell adhesive proteins, was synthesized according to the solution method and covalently coupled onto monodisperse microspheres. The biospecific activity of the RGDS-carrying microspheres was investigated by measuring the stimulatory effect of the microspheres on platelets. Large and tightly packed platelet aggregation accompanied with drastic ATP release was induced by the RGDS-carrying microspheres. It was confirmed that the specific platelet aggregation was attributed to the activity of the immobilized RGDS by comparison with those induced by other microspheres.

KW - Microsphere

KW - RGDS

KW - biospecific activity

KW - cell adhesion

KW - platelet aggregation

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Preparation Of Peptide-Carrying Microspheres With Bioactivity On Platelets

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