Pressure stimulates proliferation and DNA synthesis in rat intestinal epithelial cells

Masahiko Hirokawa; Soichiro Miura; Takeharu Shigematsu; Hideo Yoshida; Ryota Hokari; Hajime Higuchi; Iwao Kurose; Hiroyuki Kimura; Hidetsugu Saito; Toshio Nakaki; Hiromasa Ishii

doi:10.1016/S0024-3205(97)00531-6

Pressure stimulates proliferation and DNA synthesis in rat intestinal epithelial cells

Masahiko Hirokawa, Soichiro Miura, Takeharu Shigematsu, Hideo Yoshida, Ryota Hokari, Hajime Higuchi, Iwao Kurose, Hiroyuki Kimura, Hidetsugu Saito, Toshio Nakaki, Hiromasa Ishii

研究成果: Article › 査読

12 被引用数 (Scopus)

抄録

Effects of intraluminal pressure on cultured intestinal epithelial cells were assessed by measuring cell proliferation and DNA synthesis after exposure to various pressures. Pressures of 40 to 120 mm Hg promoted cell proliferation and DNA synthesis. Pressure-induced DNA synthesis was further enhanced by the addition of interleukin-2, suggesting the regulation of intestinal epithelial growth by pressure in coordination with cytokines. Pretreatment with either a phospholipase C inhibitor or protein kinase C inhibitor significantly inhibited DNA synthesis promoted by pressure and interleukin-2. This study demonstrates a novel mechanism whereby pressure regulates cell growth in intestinal epithelial cells, possibly via activation of phospholipase C and protein kinase C.

本文言語	English
ページ（範囲）	667-672
ページ数	6
ジャーナル	Life Sciences
巻	61
号	7
DOI	https://doi.org/10.1016/S0024-3205(97)00531-6
出版ステータス	Published - 1997 7月 11
外部発表	はい

ASJC Scopus subject areas

生化学、遺伝学、分子生物学（全般）
薬理学、毒性学および薬学（全般）

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10.1016/S0024-3205(97)00531-6

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title = "Pressure stimulates proliferation and DNA synthesis in rat intestinal epithelial cells",

abstract = "Effects of intraluminal pressure on cultured intestinal epithelial cells were assessed by measuring cell proliferation and DNA synthesis after exposure to various pressures. Pressures of 40 to 120 mm Hg promoted cell proliferation and DNA synthesis. Pressure-induced DNA synthesis was further enhanced by the addition of interleukin-2, suggesting the regulation of intestinal epithelial growth by pressure in coordination with cytokines. Pretreatment with either a phospholipase C inhibitor or protein kinase C inhibitor significantly inhibited DNA synthesis promoted by pressure and interleukin-2. This study demonstrates a novel mechanism whereby pressure regulates cell growth in intestinal epithelial cells, possibly via activation of phospholipase C and protein kinase C.",

keywords = "Cell proliferation, Interleukin-2, Intestinal epithelial cells, Intraluminal pressure, Phospholipase C, Protein kinase C",

author = "Masahiko Hirokawa and Soichiro Miura and Takeharu Shigematsu and Hideo Yoshida and Ryota Hokari and Hajime Higuchi and Iwao Kurose and Hiroyuki Kimura and Hidetsugu Saito and Toshio Nakaki and Hiromasa Ishii",

note = "Funding Information: This study was supportedi n part by grants from the Ministry of Education, Science and Culture of Japan and from Keio University. We thank Dr. Keiichi Hishikawa for his valuable advicea nd Ms. Ruri Caroline Nakatsumif or her technicala ssistance.",

year = "1997",

month = jul,

day = "11",

doi = "10.1016/S0024-3205(97)00531-6",

language = "English",

volume = "61",

pages = "667--672",

journal = "Life Sciences",

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TY - JOUR

T1 - Pressure stimulates proliferation and DNA synthesis in rat intestinal epithelial cells

AU - Hirokawa, Masahiko

AU - Miura, Soichiro

AU - Shigematsu, Takeharu

AU - Yoshida, Hideo

AU - Hokari, Ryota

AU - Higuchi, Hajime

AU - Kurose, Iwao

AU - Kimura, Hiroyuki

AU - Saito, Hidetsugu

AU - Nakaki, Toshio

AU - Ishii, Hiromasa

N1 - Funding Information: This study was supportedi n part by grants from the Ministry of Education, Science and Culture of Japan and from Keio University. We thank Dr. Keiichi Hishikawa for his valuable advicea nd Ms. Ruri Caroline Nakatsumif or her technicala ssistance.

PY - 1997/7/11

Y1 - 1997/7/11

N2 - Effects of intraluminal pressure on cultured intestinal epithelial cells were assessed by measuring cell proliferation and DNA synthesis after exposure to various pressures. Pressures of 40 to 120 mm Hg promoted cell proliferation and DNA synthesis. Pressure-induced DNA synthesis was further enhanced by the addition of interleukin-2, suggesting the regulation of intestinal epithelial growth by pressure in coordination with cytokines. Pretreatment with either a phospholipase C inhibitor or protein kinase C inhibitor significantly inhibited DNA synthesis promoted by pressure and interleukin-2. This study demonstrates a novel mechanism whereby pressure regulates cell growth in intestinal epithelial cells, possibly via activation of phospholipase C and protein kinase C.

AB - Effects of intraluminal pressure on cultured intestinal epithelial cells were assessed by measuring cell proliferation and DNA synthesis after exposure to various pressures. Pressures of 40 to 120 mm Hg promoted cell proliferation and DNA synthesis. Pressure-induced DNA synthesis was further enhanced by the addition of interleukin-2, suggesting the regulation of intestinal epithelial growth by pressure in coordination with cytokines. Pretreatment with either a phospholipase C inhibitor or protein kinase C inhibitor significantly inhibited DNA synthesis promoted by pressure and interleukin-2. This study demonstrates a novel mechanism whereby pressure regulates cell growth in intestinal epithelial cells, possibly via activation of phospholipase C and protein kinase C.

KW - Cell proliferation

KW - Interleukin-2

KW - Intestinal epithelial cells

KW - Intraluminal pressure

KW - Phospholipase C

KW - Protein kinase C

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U2 - 10.1016/S0024-3205(97)00531-6

DO - 10.1016/S0024-3205(97)00531-6

M3 - Article

C2 - 9252241

AN - SCOPUS:0030850809

VL - 61

SP - 667

EP - 672

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 7

ER -

Pressure stimulates proliferation and DNA synthesis in rat intestinal epithelial cells

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