Pressure stimulates proliferation and DNA synthesis in rat intestinal epithelial cells

Masahiko Hirokawa, Soichiro Miura, Takeharu Shigematsu, Hideo Yoshida, Ryota Hokari, Hajime Higuchi, Iwao Kurose, Hiroyuki Kimura, Hidetsugu Saito, Toshio Nakaki, Hiromasa Ishii

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Effects of intraluminal pressure on cultured intestinal epithelial cells were assessed by measuring cell proliferation and DNA synthesis after exposure to various pressures. Pressures of 40 to 120 mm Hg promoted cell proliferation and DNA synthesis. Pressure-induced DNA synthesis was further enhanced by the addition of interleukin-2, suggesting the regulation of intestinal epithelial growth by pressure in coordination with cytokines. Pretreatment with either a phospholipase C inhibitor or protein kinase C inhibitor significantly inhibited DNA synthesis promoted by pressure and interleukin-2. This study demonstrates a novel mechanism whereby pressure regulates cell growth in intestinal epithelial cells, possibly via activation of phospholipase C and protein kinase C.

本文言語English
ページ(範囲)667-672
ページ数6
ジャーナルLife Sciences
61
7
DOI
出版ステータスPublished - 1997 7月 11
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 薬理学、毒性学および薬学(全般)

フィンガープリント

「Pressure stimulates proliferation and DNA synthesis in rat intestinal epithelial cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル