Printed low-cost microfluidic analytical devices based on a transparent substrate

Shogo Fujisaki, Hiroyuki Shibata, Kentaro Yamada, Koji Suzuki, Daniel Citterio

研究成果: Article

1 引用 (Scopus)

抄録

This work describes the development of a microfluidic analytical device prepared on a transparent OHP film substrate, named the microfluidic transparent film-based analytical device (μTFAD). Printing technologies including wax printing for microchannel patterning and inkjet printing for chemical assay component deposition have been employed for the μTFAD fabrication. The fully printed μTFAD allowed gravity-assisted pump-free transportation of the sample liquid (50 μL) and an absorbance measurement-based iron ion (Fe 2+ ) assay using nitroso-PSAP as the colorimetric reagent within a wax-patterned microfluidic structure. By measuring absorbance values at the Fe 2+ -nitroso-PSAP complex-specific wavelength (756 nm), a response curve with a linear range of 0-200 μM was obtained. The limit of detection (1.18 μM) obtained with the proposed μTFADs was comparable to the results achieved with a conventional 96-well microplate assay (0.92 μM) and lower than that in the case of digital colour analysis-assisted filter paper spot tests (7.71 μM) or the absorbance analysis of refractive index-matched translucent filter paper spots (37.2 μM). In addition, highly selective Fe 2+ detection has been achieved in the presence of potentially interfering metal ions (Cu 2+ , Co 2+ , Ni 2+ ) without the use of any masking reagents, owing to the selection of the target complex-specific wavelength in the absorbance measurement on μTFADs.

元の言語English
ページ(範囲)2746-2754
ページ数9
ジャーナルAnalyst
144
発行部数8
DOI
出版物ステータスPublished - 2019 4 21

Fingerprint

Lab-On-A-Chip Devices
Printing
absorbance
Microfluidics
Waxes
Costs and Cost Analysis
substrate
Assays
Equipment and Supplies
assay
Substrates
wax
cost
Ions
Costs
Refractometry
Gravitation
filter
wavelength
Wavelength

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

これを引用

Printed low-cost microfluidic analytical devices based on a transparent substrate. / Fujisaki, Shogo; Shibata, Hiroyuki; Yamada, Kentaro; Suzuki, Koji; Citterio, Daniel.

:: Analyst, 巻 144, 番号 8, 21.04.2019, p. 2746-2754.

研究成果: Article

Fujisaki, S, Shibata, H, Yamada, K, Suzuki, K & Citterio, D 2019, 'Printed low-cost microfluidic analytical devices based on a transparent substrate', Analyst, 巻. 144, 番号 8, pp. 2746-2754. https://doi.org/10.1039/c8an02304b
Fujisaki, Shogo ; Shibata, Hiroyuki ; Yamada, Kentaro ; Suzuki, Koji ; Citterio, Daniel. / Printed low-cost microfluidic analytical devices based on a transparent substrate. :: Analyst. 2019 ; 巻 144, 番号 8. pp. 2746-2754.
@article{cfaae2d95a544475b3b4fed431fe5629,
title = "Printed low-cost microfluidic analytical devices based on a transparent substrate",
abstract = "This work describes the development of a microfluidic analytical device prepared on a transparent OHP film substrate, named the microfluidic transparent film-based analytical device (μTFAD). Printing technologies including wax printing for microchannel patterning and inkjet printing for chemical assay component deposition have been employed for the μTFAD fabrication. The fully printed μTFAD allowed gravity-assisted pump-free transportation of the sample liquid (50 μL) and an absorbance measurement-based iron ion (Fe 2+ ) assay using nitroso-PSAP as the colorimetric reagent within a wax-patterned microfluidic structure. By measuring absorbance values at the Fe 2+ -nitroso-PSAP complex-specific wavelength (756 nm), a response curve with a linear range of 0-200 μM was obtained. The limit of detection (1.18 μM) obtained with the proposed μTFADs was comparable to the results achieved with a conventional 96-well microplate assay (0.92 μM) and lower than that in the case of digital colour analysis-assisted filter paper spot tests (7.71 μM) or the absorbance analysis of refractive index-matched translucent filter paper spots (37.2 μM). In addition, highly selective Fe 2+ detection has been achieved in the presence of potentially interfering metal ions (Cu 2+ , Co 2+ , Ni 2+ ) without the use of any masking reagents, owing to the selection of the target complex-specific wavelength in the absorbance measurement on μTFADs.",
author = "Shogo Fujisaki and Hiroyuki Shibata and Kentaro Yamada and Koji Suzuki and Daniel Citterio",
year = "2019",
month = "4",
day = "21",
doi = "10.1039/c8an02304b",
language = "English",
volume = "144",
pages = "2746--2754",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "8",

}

TY - JOUR

T1 - Printed low-cost microfluidic analytical devices based on a transparent substrate

AU - Fujisaki, Shogo

AU - Shibata, Hiroyuki

AU - Yamada, Kentaro

AU - Suzuki, Koji

AU - Citterio, Daniel

PY - 2019/4/21

Y1 - 2019/4/21

N2 - This work describes the development of a microfluidic analytical device prepared on a transparent OHP film substrate, named the microfluidic transparent film-based analytical device (μTFAD). Printing technologies including wax printing for microchannel patterning and inkjet printing for chemical assay component deposition have been employed for the μTFAD fabrication. The fully printed μTFAD allowed gravity-assisted pump-free transportation of the sample liquid (50 μL) and an absorbance measurement-based iron ion (Fe 2+ ) assay using nitroso-PSAP as the colorimetric reagent within a wax-patterned microfluidic structure. By measuring absorbance values at the Fe 2+ -nitroso-PSAP complex-specific wavelength (756 nm), a response curve with a linear range of 0-200 μM was obtained. The limit of detection (1.18 μM) obtained with the proposed μTFADs was comparable to the results achieved with a conventional 96-well microplate assay (0.92 μM) and lower than that in the case of digital colour analysis-assisted filter paper spot tests (7.71 μM) or the absorbance analysis of refractive index-matched translucent filter paper spots (37.2 μM). In addition, highly selective Fe 2+ detection has been achieved in the presence of potentially interfering metal ions (Cu 2+ , Co 2+ , Ni 2+ ) without the use of any masking reagents, owing to the selection of the target complex-specific wavelength in the absorbance measurement on μTFADs.

AB - This work describes the development of a microfluidic analytical device prepared on a transparent OHP film substrate, named the microfluidic transparent film-based analytical device (μTFAD). Printing technologies including wax printing for microchannel patterning and inkjet printing for chemical assay component deposition have been employed for the μTFAD fabrication. The fully printed μTFAD allowed gravity-assisted pump-free transportation of the sample liquid (50 μL) and an absorbance measurement-based iron ion (Fe 2+ ) assay using nitroso-PSAP as the colorimetric reagent within a wax-patterned microfluidic structure. By measuring absorbance values at the Fe 2+ -nitroso-PSAP complex-specific wavelength (756 nm), a response curve with a linear range of 0-200 μM was obtained. The limit of detection (1.18 μM) obtained with the proposed μTFADs was comparable to the results achieved with a conventional 96-well microplate assay (0.92 μM) and lower than that in the case of digital colour analysis-assisted filter paper spot tests (7.71 μM) or the absorbance analysis of refractive index-matched translucent filter paper spots (37.2 μM). In addition, highly selective Fe 2+ detection has been achieved in the presence of potentially interfering metal ions (Cu 2+ , Co 2+ , Ni 2+ ) without the use of any masking reagents, owing to the selection of the target complex-specific wavelength in the absorbance measurement on μTFADs.

UR - http://www.scopus.com/inward/record.url?scp=85064154052&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85064154052&partnerID=8YFLogxK

U2 - 10.1039/c8an02304b

DO - 10.1039/c8an02304b

M3 - Article

C2 - 30869086

AN - SCOPUS:85064154052

VL - 144

SP - 2746

EP - 2754

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 8

ER -