Production of functional oocytes requires maternally expressed PIWI genes and piRNAs in golden hamsters

Hidetoshi Hasuwa, Yuka W. Iwasaki, Wan Kin Au Yeung, Kyoko Ishino, Harumi Masuda, Hiroyuki Sasaki, Haruhiko Siomi

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Many animals have a conserved adaptive genome defence system known as the Piwi-interacting RNA (piRNA) pathway, which is essential for germ cell development and function. Disruption of individual mouse Piwi genes results in male but not female sterility, leading to the assumption that PIWI genes play little or no role in mammalian oocytes. Here, we report the generation of PIWI-defective golden hamsters, which have defects in the production of functional oocytes. The mechanisms involved vary among the hamster PIWI genes, whereby the lack of PIWIL1 has a major impact on gene expression, including hamster-specific young transposon de-silencing, whereas PIWIL3 deficiency has little impact on gene expression in oocytes, although DNA methylation was reduced to some extent in PIWIL3-deficient oocytes. Our findings serve as the foundation for developing useful models to study the piRNA pathway in mammalian oocytes, including humans.

本文言語English
ページ(範囲)1002-1012
ページ数11
ジャーナルNature Cell Biology
23
9
DOI
出版ステータスPublished - 2021 9月

ASJC Scopus subject areas

  • 細胞生物学

フィンガープリント

「Production of functional oocytes requires maternally expressed PIWI genes and piRNAs in golden hamsters」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル