@inbook{e4bfc0b909db4115be46a07df8c4a9b7,
title = "Profiling open chromatin structure in the ovarian somatic cells using ATAC-seq",
abstract = "The assay for transposase-accessible chromatin using sequencing (ATAC-seq) was recently established as a method to profile open chromatin, which overcomes the sample size limitations of the alternative methods DNase/MNase-seq. To investigate the role of Piwi in heterochromatin formation around transposable element loci, we have used ATAC-seq to examine chromatin accessibility at target transposable elements in a Drosophila cultured cell line, ovarian somatic cells (OSCs). In this chapter, we describe our method to profile open chromatin structure in OSCs using ATAC-seq.",
keywords = "ATAC-seq, Chromatin accessibility, Drosophila, Heterochromatin, OSC, Small RNA, Transposons",
author = "Kensaku Murano and Iwasaki, {Yuka W.} and Haruhiko Siomi",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media LLC.",
year = "2018",
doi = "10.1007/978-1-4939-7339-2_11",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "165--177",
booktitle = "Methods in Molecular Biology",
}