Proliferation and adhesion of L929 fibroblasts on surface with different microtopography

Yuta Kurashina, Shogo Miyata, Jun Komotori, Tadayoshi Koyama

研究成果: Conference contribution

抄録

Three types of 316L stainless steel surface with different topography were prepared by a Fine Particle Peening (FPP) treatment using titania, silica and alumina shot particles and analyzed the cell proliferation and cell-scaffold interaction. FPP-treated surface with titania and silica particles had micro asperities at low frequency. On the other hand, the alumina treated surface had micro asperities at high frequency. L929 fibroblasts were seeded on these specimens and then the number of cells was counted after 72 hours of culturing. The FPP-treated surfaces showed good cell proliferation comparing to polished surface. This indicates that micro asperities formed on the surface encourage cell adhesion. Cell adhesion behavior was evaluated by a scanning electron microscope (SEM) and a fluorescence microscope. Dense filopodia were observed when cells cultured on the FPP-treated surface. This means that FPP treatment enhances cell adhesion and proliferation. The number of cells observed on the FPP-treated surface depended on the shape of asperities formed by FPP treatment; the highest cell counts were obtained on alumina treated surface. This is because cell migration was not inhibited by the shape of alumina treated surface asperities.

元の言語English
ホスト出版物のタイトルMaterials Research Society Symposium Proceedings
出版者Materials Research Society
1648
DOI
出版物ステータスPublished - 2014
イベント2013 MRS Fall Meeting - Boston, MA, United States
継続期間: 2013 12 12013 12 6

Other

Other2013 MRS Fall Meeting
United States
Boston, MA
期間13/12/113/12/6

Fingerprint

fibroblasts
Fibroblasts
peening
Shot peening
adhesion
Adhesion
Aluminum Oxide
cells
Cell adhesion
Cell proliferation
Alumina
aluminum oxides
Silicon Dioxide
titanium
Titanium
Silica
silicon dioxide
Stainless Steel
Scaffolds (biology)
cultured cells

ASJC Scopus subject areas

  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)
  • Condensed Matter Physics

これを引用

Kurashina, Y., Miyata, S., Komotori, J., & Koyama, T. (2014). Proliferation and adhesion of L929 fibroblasts on surface with different microtopography. : Materials Research Society Symposium Proceedings (巻 1648). Materials Research Society. https://doi.org/10.1557/opl.2014.228

Proliferation and adhesion of L929 fibroblasts on surface with different microtopography. / Kurashina, Yuta; Miyata, Shogo; Komotori, Jun; Koyama, Tadayoshi.

Materials Research Society Symposium Proceedings. 巻 1648 Materials Research Society, 2014.

研究成果: Conference contribution

Kurashina, Y, Miyata, S, Komotori, J & Koyama, T 2014, Proliferation and adhesion of L929 fibroblasts on surface with different microtopography. : Materials Research Society Symposium Proceedings. 巻. 1648, Materials Research Society, 2013 MRS Fall Meeting, Boston, MA, United States, 13/12/1. https://doi.org/10.1557/opl.2014.228
Kurashina Y, Miyata S, Komotori J, Koyama T. Proliferation and adhesion of L929 fibroblasts on surface with different microtopography. : Materials Research Society Symposium Proceedings. 巻 1648. Materials Research Society. 2014 https://doi.org/10.1557/opl.2014.228
Kurashina, Yuta ; Miyata, Shogo ; Komotori, Jun ; Koyama, Tadayoshi. / Proliferation and adhesion of L929 fibroblasts on surface with different microtopography. Materials Research Society Symposium Proceedings. 巻 1648 Materials Research Society, 2014.
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