Promoter activity of the 5′-flanking regions of medaka fish soluble guanylate cyclase α1 and β subunit genes

Takehiro Yamamoto, Norio Suzuki

研究成果: Article査読

10 被引用数 (Scopus)

抄録

We examined the spatial expression pattern of medaka fish (Oryzias latipes) soluble guanylate cyclase α1 and β1 subunit genes, OlGCS-α1 and OIGCS-β1, and characterized the 5′-flanking region required for expression of both genes by introducing various promoter-luciferase fusion-gene constructs into COS-1 cells and medaka fish embryos. The OlGCS-α1 and OIGCS-β1 gene transcripts were detected in whole brain and kidney in 7-day and 9-day embryos. Primer-extension analysis demonstrated that there were no differences among various adult organs (brain, eye, kidney, ovary and testis) in the transcription start site of the OIGCS-α1 and OIGCS-β1 genes. Neither gene contained the functional TATA box within its 5′-flanking region, and the basal promoter activity was found between nucleotides +33 and +42 in the OIGCS-α1 gene and between nucleotides + 146 and + 155 in the OIGCS-β1 gene. In the assay of medaka fish embryos, the 5′-flanking region of the OIGCS-β1 gene exhibited lower promoter activity than that of the OIGCS-α1 gene. In the experiments on dual-luciferase fusion-gene constructs, the 5′-flanking region of the OIGCS-α1 gene connected to the 5′-flanking region of the OIGCS-β1 gene was introduced into medaka fish embryos, and the 5′-flanking regions of both subunit genes were shown to mutually influence each other's promoter activity.

本文言語English
ページ(範囲)337-345
ページ数9
ジャーナルBiochemical Journal
361
2
DOI
出版ステータスPublished - 2002 1 15

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

フィンガープリント 「Promoter activity of the 5′-flanking regions of medaka fish soluble guanylate cyclase α<sub>1</sub> and β subunit genes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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