TY - JOUR
T1 - Promutagen activation by Vicia faba
T2 - An assay based on the induction of sister-chromatid exchanges in Chinese hamster ovary cells
AU - Takehisa, Shin
AU - Kanaya, Nobuhiro
AU - Rieger, Rigomar
PY - 1988/2
Y1 - 1988/2
N2 - Plant activation of promutagens was studied using Vicia faba S10 (in vitro activation) and the extracts prepared from promutagen-treated roots of Vicia faba (in vivo activation). The induction of sister-chromatid exchanges in Chinese hamster ovary cells was used as an endpoint to evaluate the cytogenetic effects of promutagens activated by Vicia faba. Cyclophosphamide and ethyl alcohol were activated both by Vicia S10 and by the Vicia extracts, and their activation resulted in an increase in SCEs. Benzo[a]pyrene, 2-aminofluorene, and maleic hydrazide were not activated. Aniline was activated, but without effect on the induction of SCEs. The activation capacity in vitro and in vivo of Vicia faba not very pronounced, except for the activation of ethyl alcohol, when compared with that of rat-liver S9, and showed differences in activation for the 6 chemical agents tested.
AB - Plant activation of promutagens was studied using Vicia faba S10 (in vitro activation) and the extracts prepared from promutagen-treated roots of Vicia faba (in vivo activation). The induction of sister-chromatid exchanges in Chinese hamster ovary cells was used as an endpoint to evaluate the cytogenetic effects of promutagens activated by Vicia faba. Cyclophosphamide and ethyl alcohol were activated both by Vicia S10 and by the Vicia extracts, and their activation resulted in an increase in SCEs. Benzo[a]pyrene, 2-aminofluorene, and maleic hydrazide were not activated. Aniline was activated, but without effect on the induction of SCEs. The activation capacity in vitro and in vivo of Vicia faba not very pronounced, except for the activation of ethyl alcohol, when compared with that of rat-liver S9, and showed differences in activation for the 6 chemical agents tested.
KW - Chinese hamster ovary cells
KW - Cyclophosphamide
KW - Ethanol
KW - Promutagen activation
KW - SCE induction
KW - Vicia faba
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U2 - 10.1016/0027-5107(88)90093-0
DO - 10.1016/0027-5107(88)90093-0
M3 - Article
C2 - 3340085
AN - SCOPUS:0023958555
VL - 197
SP - 195
EP - 205
JO - Mutation Research
JF - Mutation Research
SN - 0027-5107
IS - 2
ER -