Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow

Satoru Morikawa, Yo Mabuchi, Yoshiaki Kubota, Yasuo Nagai, Kunimichi Niibe, Emi Hiratsu, Sadafumi Suzuki, Chikako Miyauchi-Hara, Narihito Nagoshi, Takehiko Sunabori, Shigeto Shimmura, Atsushi Miyawaki, Taneaki Nakagawa, Toshio Suda, Hideyuki Okano, Yumi Matsuzaki

研究成果: Article査読

540 被引用数 (Scopus)

抄録

Mesenchymal stem cells (MSCs) are defined as cells that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identified in vivo and little is known about their physiological roles or even their exact tissue location. In this study, we used phenotypic, morphological, and functional criteria to identify and prospectively isolate a subset of MSCs (PDGFRα+Sca-1+CD45 -TER119-) from adult mouse bone marrow. Individual MSCs generated colonies at a high frequency and could differentiate into hematopoietic niche cells, osteoblasts, and adipocytes after in vivo transplantation. Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities.

本文言語English
ページ(範囲)2483-2496
ページ数14
ジャーナルJournal of Experimental Medicine
206
11
DOI
出版ステータスPublished - 2009 10 26

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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