Protein phosphatase complex PP5/PPP2R3C dephosphorylates P-glycoprotein/ABCB1 and down-regulates the expression and function

Kazuhiro Katayama, Miho Yamaguchi, Kohji Noguchi, Yoshikazu Sugimoto

研究成果: Article

16 被引用数 (Scopus)

抄録

P-glycoprotein (P-gp)/ABCB1 is a key molecule of multidrug resistance in cancer. Protein phosphatase (PP) 2A, regulatory subunit B, gamma (PPP2R3C), which is a regulatory subunit of PP2A and PP5, was identified as a binding candidate to P-gp. Immunoprecipitation-western blotting revealed that PP5 and PPP2R3C were coprecipitated with P-gp, while PP2A was not. PP5/PPP2R3C dephosphorylated protein kinase A/protein kinase C-phosphorylation of P-gp. Knockdown of PP5 and/or PPP2R3C increased P-gp expression and lowered the sensitivity to vincristine and doxorubicin. Consequently, our results indicate that PP5/PPP2R3C negatively regulates P-gp expression and function.

本文言語English
ページ(範囲)124-131
ページ数8
ジャーナルCancer Letters
345
1
DOI
出版ステータスPublished - 2014 4 1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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