Protein phosphorylation involved in the gene expression of the hydrogen sulphide producing enzyme cystathionine γ-lyase in the pancreatic β-cell

Shigeki Taniguchi, Toshihide Kimura, Tatsuhito Umeki, Yuka Kimura, Hideo Kimura, Isao Ishii, Norimichi Itoh, Yasuhito Naito, Hideyuki Yamamoto, Ichiro Niki

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Cystathionine γ-lyase (CSE) is one of the major enzymes for the production of hydrogen sulphide (H 2S), a multifunctional gasotransmitter in the pancreatic β-cell. We examined the mechanisms by which glucose induces CSE expression in mouse pancreatic islets and the insulin-secreting cell line MIN6. CSE expression was increased by anti-diabetic sulphonylureas, and decreased by the ATP-sensitive K +-channel opener diazoxide and the voltage-dependent Ca 2+ channel blocker nitrendipine. Application of the synthetic inhibitors of protein kinases revealed the involvement of Ca 2+/calmodulin-dependent protein kinase (CaMK) II and extracellular signal-regulated protein kinase (ERK) in glucose- and thapsigargin-induced CSE expression. The CaMK IIδ knockdown also suppressed CSE expression. Knockdown of the transcription factors Sp1 and Elk1, both of which can be phosphorylated by ERK, blunted CSE expression. By a reporter assay, we found Sp1 may directly and Elk1 may indirectly regulate CSE expression. These findings suggest Ca 2+-dependent CSE expression may be mediated via protein phosphorylation of Sp1 and Elk1 in pancreatic β-cells.

本文言語English
ページ(範囲)31-38
ページ数8
ジャーナルMolecular and Cellular Endocrinology
350
1
DOI
出版ステータスPublished - 2012 3 5

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 内分泌学

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