Proteome analysis of focal adhesion kinase (FAK)-overexpressing cells

Ryoko Utsubo, Yoshiko Sonoda, Reiko Takahashi, Shiro Iijima, Eriko Aizu-Yokota, Tadashi Kasahara

研究成果: Article査読

3 被引用数 (Scopus)

抄録

We established several focal adhesion kinase (FAK) cDNA-transfected cells and found that FAK-transfected HL-60 (HL-60/FAK) cells are resistant to apoptosis induced with hydrogen peroxide, etoposide and radiation compared with the parental HL-60 or the vector-transfected (HL-60/Vect) cells. We carried out proteome analysis to study the mechanism of resistance to apoptosis in HL-60/FAK cells. Among 300 spots resolved in two-dimensional gels, ca. 10% of them were significantly increased in HL-60/FAK cells compared with HL-60/Vect cells, whereas ca. 2% of them were decreased or disappeared. These proteins were performed for further analysis by Western blots or N-terminal sequencing or mass spectrometry. Increased proteins included stress proteins such as hsp90, ribosomal proteins, and antioxidant enzymes such as peroxyredoxin 2. Some of these proteins are assumed to contribute to the antiapoptotic action of FAK.

本文言語English
ページ(範囲)1735-1741
ページ数7
ジャーナルBiological and Pharmaceutical Bulletin
27
11
DOI
出版ステータスPublished - 2004 11月

ASJC Scopus subject areas

  • 薬理学
  • 薬科学

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