Proteomic analysis of α-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor complexes

Myoung Goo Kang, Mutsuo Nuriya, Yurong Guo, Kevin D. Martindale, Daniel Z. Lee, Richard L. Huganir

研究成果: Article査読

23 被引用数 (Scopus)

抄録

The AMPA receptor (AMPA-R) is a major excitatory neurotransmitter receptor in the brain. Identifying and characterizing the neuronal proteins interacting with AMPA-Rs have provided important information about the molecular mechanisms underlying synaptic transmission and plasticity. In this study, to identify more AMPA-R interactors in vivo, we performed proteomic analyses of AMPA-R complexes from the brain.AMPA-Rcomplexes were isolated from the brain through various combinations of biochemical techniques for solubilization, enrichment, and immunoprecipitation. Mass spectrometry analyses of these isolated complexes identified several novel components of the AMPA-R complexes as well as some previously identified components. The identification of these novel components helps to further define the complex mechanisms involved in the regulation of AMPA receptor function and synaptic plasticity.

本文言語English
ページ(範囲)28632-28645
ページ数14
ジャーナルJournal of Biological Chemistry
287
34
DOI
出版ステータスPublished - 2012 8 17

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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